publication . Article . 2016

Physical determinants of vesicle mobility and supply at a central synapse.

Jason Seth Rothman; Laszlo Kocsis; Etienne Herzog; Zoltan Nusser; Robin Angus Silver;
Open Access English
  • Published: 19 Aug 2016
  • Country: United Kingdom
Abstract
Encoding continuous sensory variables requires sustained synaptic signalling. At several sensory synapses, rapid vesicle supply is achieved via highly mobile vesicles and specialized ribbon structures, but how this is achieved at central synapses without ribbons is unclear. Here we examine vesicle mobility at excitatory cerebellar mossy fibre synapses which sustain transmission over a broad frequency bandwidth. Fluorescent recovery after photobleaching in slices from VGLUT1Venus knock-in mice reveal 75% of VGLUT1-containing vesicles have a high mobility, comparable to that at ribbon synapses. Experimentally constrained models establish hydrodynamic interactions ...
Subjects
free text keywords: Neuroscience, Research Article, synaptic transmission, vesicle mobility, diffusion, hydrodynamic interactions, active zone, vesicle supply, Mouse, Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, HARD-SPHERE SUSPENSIONS, MOTOR-NERVE TERMINALS, TIME SELF-DIFFUSION, IN-VIVO, RIBBON SYNAPSE, HIPPOCAMPAL SYNAPSES, VESICULAR RELEASE, AUDITORY SYNAPSE, BROWNIAN-MOTION, AMPA RECEPTORS
Funded by
WT
Project
  • Funder: Wellcome Trust (WT)
,
WT| The cellular basis of information processing in a cerebellar microcircuit
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 203048
  • Funding stream: Cellular and Molecular Neuroscience
,
EC| MOLECMAP
Project
MOLECMAP
Quantitative Molecular Map of the Neuronal Surface
  • Funder: European Commission (EC)
  • Project Code: 293681
  • Funding stream: FP7 | SP2 | ERC
,
EC| NEUROGAIN
Project
NEUROGAIN
Nonlinear synaptic integration in morphologically simple and complex neurons
  • Funder: European Commission (EC)
  • Project Code: 294667
  • Funding stream: FP7 | SP2 | ERC
,
WT| Synaptic and neuronal determinants of network function: Application of new optical and computational tools.
Project
  • Funder: Wellcome Trust (WT)
  • Project Code: 095667
  • Funding stream: Neuroscience and Mental Health
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