publication . Other literature type . Article . 2002

Infection of a human hepatoma cell line by hepatitis B virus

P. Gripon; S. Rumin; S. Urban; J. Le Seyec; D. Glaise; I. Cannie; C. Guyomard; J. Lucas; C. Trepo; C. Guguen-Guillouzo;
Open Access
  • Published: 13 Nov 2002
  • Publisher: Proceedings of the National Academy of Sciences
Abstract
Among numerous established human hepatoma cell lines, none has been shown susceptible to hepatitis B virus (HBV) infection. We describe here a cell line, called HepaRG, which exhibits hepatocyte-like morphology, expresses specific hepatocyte functions, and supports HBV infection as well as primary cultures of normal human hepatocytes. Differentiation and infectability are maintained only when these cells are cultured in the presence of corticoids and dimethyl sulfoxide. The specificity of this HBV infection model was ascertained by both the neutralization capacity of HBV-envelope protein-specific antibodies and the competition with an envelope-derived peptide. H...
Subjects
Medical Subject Headings: digestive system diseases
free text keywords: Multidisciplinary
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