publication . Article . 2004

Preparation of stock solutions of macrolide and ketolide compounds for antimicrobial susceptibility tests

André Bryskier; M. M. Traczewski; Steven D. Brown; Arthur L. Barry;
Open Access
  • Published: 01 Jan 2004 Journal: Clinical Microbiology and Infection, volume 10, issue 1, pages 78-83 (issn: 1198-743X, Copyright policy)
  • Publisher: Elsevier BV
Abstract
ABSTRACTStock solutions of telithromycin, ABT-773, azithromycin, clarithromycin, erythromycin, roxithromycin and dirithromycin were each prepared with eight different combinations of solvents and diluents. Broth microdilution trays were then prepared and frozen at −60 ≥C. Standard quality control strains were evaluated periodically during a 12-week storage time. There were no significant changes in MICs with different solvents and diluents. It was concluded that the easiest approach was to dissolve each compound in water with a small volume (< 2.5 μL/mL) of glacial acetic acid added in a dropwise fashion, followed by further dilutions in deionised water.
Subjects
free text keywords: Microbiology (medical), Infectious Diseases, Ketolide, macrolide, stock solutions, susceptibility tests, General Medicine, Clarithromycin, medicine.drug, medicine, Broth microdilution, Roxithromycin, Antibacterial agent, Chromatography, Dirithromycin, Chemistry, Telithromycin, Microbiology, Erythromycin
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