publication . Article . 2004

Preparation of stock solutions of macrolide and ketolide compounds for antimicrobial susceptibility tests

André Bryskier; M. M. Traczewski; Steven D. Brown; Arthur L. Barry;
Open Access
  • Published: 01 Jan 2004 Journal: Clinical Microbiology and Infection, volume 10, issue 1, pages 78-83 (issn: 1198-743X, Copyright policy)
  • Publisher: Elsevier BV
ABSTRACTStock solutions of telithromycin, ABT-773, azithromycin, clarithromycin, erythromycin, roxithromycin and dirithromycin were each prepared with eight different combinations of solvents and diluents. Broth microdilution trays were then prepared and frozen at −60 ≥C. Standard quality control strains were evaluated periodically during a 12-week storage time. There were no significant changes in MICs with different solvents and diluents. It was concluded that the easiest approach was to dissolve each compound in water with a small volume (< 2.5 μL/mL) of glacial acetic acid added in a dropwise fashion, followed by further dilutions in deionised water.
free text keywords: Microbiology (medical), Infectious Diseases, Ketolide, macrolide, stock solutions, susceptibility tests, General Medicine, Clarithromycin, medicine.drug, medicine, Broth microdilution, Roxithromycin, Antibacterial agent, Chromatography, Dirithromycin, Chemistry, Telithromycin, Microbiology, Erythromycin
Related Organizations
Any information missing or wrong?Report an Issue