publication . Article . Other literature type . 1994

Adhesion forces between individual ligand-receptor pairs

Ernst-Ludwig Florin; Vincent T. Moy; Hermann E. Gaub;
Open Access
  • Published: 15 Apr 1994 Journal: Science, volume 264, pages 415-417 (issn: 0036-8075, eissn: 1095-9203, Copyright policy)
  • Publisher: American Association for the Advancement of Science (AAAS)
Abstract
The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 +/- 20 piconewtons for biotin and 85 +/- 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.
Subjects
free text keywords: Multidisciplinary, Biotin, chemistry.chemical_compound, chemistry, Cantilever, Ligand (biochemistry), Force spectroscopy, Avidin, biology.protein, biology, Agarose, Ligand, Adhesion, Stereochemistry, Biophysics
Related Organizations
Download fromView all 2 versions
Science
Article
Provider: UnpayWall
http://dx.doi.org/10.1126/scie...
Other literature type . 1994
Provider: Datacite
Science
Article . 1994
Provider: Crossref
Any information missing or wrong?Report an Issue