publication . Article . Other literature type . 2012

Ultraconserved elements anchor thousands of genetic markers spanning multiple evolutionary timescales.

Michael G. Harvey; Nicholas G. Crawford; Travis C. Glenn; John E. McCormack; Robb T. Brumfield; Brant C. Faircloth;

Open Access

Published: 01 Oct 2012 Journal: Systematic Biology, volume 61, pages 717-726 (issn: 1063-5157, eissn: 1076-836X,

Publisher: Oxford University Press (OUP)

Summary

Abstract

Although massively parallel sequencing has facilitated large-scale DNA sequencing, comparisons among distantly related species rely upon small portions of the genome that are easily aligned. Methods are needed to efficiently obtain comparable DNA fragments prior to massively parallel sequencing, particularly for biologists working with non- model organisms. We introduce a new class of molecular marker, anchored by ultraconserved genomic elements (UCEs), that universally enable target enrichment and sequencing of thousands of orthologous loci across species separated by hundreds of millions of years of evolution. Our analyses here focus on use of UCE markers in A...

doi: 10.1093/sysbio/sys004

Subjects

free text keywords: Phylogenomics, Molecular marker, chemistry.chemical_compound, chemistry, Genetics, Evolutionary biology, Massive parallel sequencing, Genetic marker, Phylogenetic tree, Biology, Genome, Phylogenetics, DNA sequencing

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