publication . Article . Other literature type . 2018

Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection.

Christian Tiede; Darren Tomlinson; Pawan Jolly; Estrela, Pedro;
Open Access English
  • Published: 15 Jun 2018
  • Publisher: Elsevier
  • Country: United Kingdom
Abstract
Abstract A novel Affimer-functionalised interdigitated electrode-based capacitive biosensor platform was developed for detection and estimation of Her4, a protein tumour biomarker, in undiluted serum. An anti-Her4 Affimer with a C-terminal cysteine was used to create the bio-recognition layer via self-assembly on gold interdigitated electrodes for the sensor fabrication. Electrochemical impedance spectroscopy (EIS) in the absence of redox markers was used to evaluate the sensor performance by monitoring the changes in capacitance. The Affimer sensor in buffer and in undiluted serum demonstrated high sensitivity with a broad dynamic range from 1 pM to 100 nM and ...
Subjects
free text keywords: Biosensor, Affimer, Impedance, Capacitance, serum, Her4, Biotechnology, Biophysics, Electrochemistry, Biomedical Engineering, General Medicine
Funded by
EC| PROSENSE
Project
PROSENSE
Cancer Diagnosis: Parallel Sensing of Prostate Cancer Biomarkers
  • Funder: European Commission (EC)
  • Project Code: 317420
  • Funding stream: FP7 | SP3 | PEOPLE
,
EC| BlaC E-assay
Project
BlaC E-assay
Portable electrochemical assay system for on-chip quantitative estimation of bladder cancer biomarkers in real samples
  • Funder: European Commission (EC)
  • Project Code: 655176
  • Funding stream: H2020 | MSCA-IF-EF-ST
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