publication . Article . Other literature type . 2012

Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3′-end-seq

Zhe Ji; Mainul Hoque; Nigel Rust; Jane Mellor; Bin Tian; Michael O. Hengartner; Andre Furger; Cathy Browne; Helen E. Sharpe; Simon Haenni; ...
Open Access English
  • Published: 01 Jul 2012 Journal: Nucleic Acids Research, volume 40, issue 13, pages 6,304-6,318 (issn: 0305-1048, eissn: 1362-4962, Copyright policy)
  • Publisher: Oxford University Press
Abstract
Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and purification of nuclei from major tissues of post-embryonic animals by fluorescence-activated nuclei sorting (FANS), followed by deep sequencing of linearly amplified 3'-end regions of transcripts (3'-end-seq). We employed these approaches to compile the transcriptome of the developed C. elegans intestine and used this to analyse tissue-specific cleavage and polyaden...
Subjects
free text keywords: RNA, Genetics, Institute of Molecular Life Sciences, 570 Life sciences; biology, Gene expression, Deep sequencing, Cell nucleus, medicine.anatomical_structure, medicine, Gene, Cell biology, Caenorhabditis elegans, biology.organism_classification, biology, Molecular biology, Transcriptome, Sequence analysis, Polyadenylation
Funded by
SNSF| Apoptosis in the nematode C. elegans
Project
  • Funder: Swiss National Science Foundation (SNSF)
  • Project Code: 3100A0B-128675
  • Funding stream: Project funding | Project funding (Div. I-III)
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