publication . Article . Other literature type . 2008

High-throughput microplate technique for enzymatic hydrolysis of lignocellulosic biomass.

Shishir Chundawat;
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  • Published: 15 Apr 2008 Journal: Biotechnology and Bioengineering, volume 99, pages 1,281-1,294 (issn: 0006-3592, eissn: 1097-0290, Copyright policy)
  • Publisher: Wiley
Abstract
Several factors will influence the viability of a biochemical platform for manufacturing lignocellulosic based fuels and chemicals, for example, genetically engineer- ing energy crops, reducing pre-treatment severity, and minimizing enzyme loading. Past research on biomass con- version has focused largely on acid based pre-treatment technologies that fractionate lignin and hemicellulose from cellulose. However, for alkaline based (e.g., AFEX) and other lower severity pre-treatments it becomes critical to co-hydrolyze cellulose and hemicellulose using an optimized enzyme cocktail. Lignocellulosics are appropriate substrates to assess hydrolytic activity of enzyme...
Subjects
Medical Subject Headings: food and beverages
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, Bioengineering
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