publication . Other literature type . Article . 2001

Role for a bidentate ribonuclease in the initiation step of RNA interference

Amy Caudy;
Restricted
  • Published: 01 Jan 2001
  • Publisher: Springer Science and Business Media LLC
Abstract
RNA interference (RNAi) is the mechanism through which double-stranded RNAs silence cognate genes1,2,3,4,5. In plants, this can occur at both the transcriptional and the post-transcriptional levels1,2,5; however, in animals, only post-transcriptional RNAi has been reported to date. In both plants and animals, RNAi is characterized by the presence of RNAs of about 22 nucleotides in length that are homologous to the gene that is being suppressed6,7,8. These 22-nucleotide sequences serve as guide sequences that instruct a multicomponent nuclease, RISC, to destroy specific messenger RNAs6. Here we identify an enzyme, Dicer, which can produce putative guide RNAs. Dic...
Subjects
Medical Subject Headings: fungifood and beverages
Abstract
RNA interference (RNAi) is the mechanism through which double-stranded RNAs silence cognate genes1,2,3,4,5. In plants, this can occur at both the transcriptional and the post-transcriptional levels1,2,5; however, in animals, only post-transcriptional RNAi has been reported to date. In both plants and animals, RNAi is characterized by the presence of RNAs of about 22 nucleotides in length that are homologous to the gene that is being suppressed6,7,8. These 22-nucleotide sequences serve as guide sequences that instruct a multicomponent nuclease, RISC, to destroy specific messenger RNAs6. Here we identify an enzyme, Dicer, which can produce putative guide RNAs. Dic...
Subjects
Medical Subject Headings: fungifood and beverages
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue