publication . Article . Other literature type . 2011

Transcriptome Sequencing Demonstrates that Human Papillomavirus Is Not Active in Cutaneous Squamous Cell Carcinoma

Arron, Sarah T.; Graham Ruby, J.; Dybbro, Eric; Ganem, Don; DeRisi, Joseph L.;
Open Access
  • Published: 01 Aug 2011
  • Publisher: eScholarship, University of California
  • Country: Mexico
Abstract
β-Human papillomavirus (β-HPV) DNA is present in some cutaneous squamous cell carcinomas (cuSCCs), but no mechanism of carcinogenesis has been determined. We used ultra-high-throughput sequencing of the cancer transcriptome to assess whether papillomavirus transcripts are present in these cancers. In all, 67 cuSCC samples were assayed for β-HPV DNA by PCR, and viral loads were measured with type-specific quantitative PCR. A total of 31 SCCs were selected for whole transcriptome sequencing. Transcriptome libraries were prepared in parallel from the HPV18-positive HeLa cervical cancer cell line and HPV16-positive primary cervical and periungual SCCs. Of the tumors...
Subjects
Medical Subject Headings: virusesvirus diseases
free text keywords: Adult, Aged, and over, Betapapillomavirus, Carcinoma, Squamous Cell, Cell Transformation, Viral, DNA, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, HeLa Cells, Human papillomavirus 16, Human papillomavirus 18, Humans, Male, Middle Aged, Papillomavirus Infections, Skin Neoplasms, Uterine Cervical Neoplasms, Viral Load, Clinical Sciences, Oncology and Carcinogenesis, Dermatology & Venereal Diseases, Article, Cell Biology, Biochemistry, Molecular Biology, Dermatology, Carcinogenesis, medicine.disease_cause, medicine, Real-time polymerase chain reaction, Koilocyte, HeLa, biology.organism_classification, biology, Transcriptome, Cancer, medicine.disease
Funded by
NIH| Clinical and Translational Science Institute
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5KL2RR024130-02
  • Funding stream: NATIONAL CENTER FOR RESEARCH RESOURCES
62 references, page 1 of 5

Alam, M, Caldwell, JB, Eliezri, YD. Human papillomavirus-associated digital squamous cell carcinoma: literature review and report of 21 new cases. J Am Acad Dermatol. 2003; 48: 385-93 [PubMed]

Altschul, SF, Gish, W, Miller, W, Myers, EW, Lipman, DJ. Basic local alignment search tool. J Mol Biol. 1990; 215: 403-10 [OpenAIRE] [PubMed]

Antonsson, A, Forslund, O, Ekberg, H, Sterner, G, Hansson, BG. The ubiquity and impressive genomic diversity of human skin papillomaviruses suggest a commensalic nature of these viruses. J Virol. 2000; 74: 11636-41 [OpenAIRE] [PubMed]

Asgari, MM, Kiviat, NB, Critchlow, CW, Stern, JE, Argenyi, ZB, Raugi, GJ. Detection of human papillomavirus DNA in cutaneous squamous cell carcinoma among immunocompetent individuals. J Invest Dermatol. 2008; 128: 1409-17 [OpenAIRE] [PubMed]

Bauer, HM, Ting, Y, Greer, CE, Chambers, JC, Tashiro, CJ, Chimera, J. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA. 1991; 265: 472-7 [PubMed]

Benson, DA, Karsch-Mizrachi, I, Lipman, DJ, Ostell, J, Sayers, EW. GenBank. Nucleic Acids Res. 2009; 37: D26-31 [OpenAIRE] [PubMed]

Berkhout, RJ, Bouwes Bavinck, JN, ter Schegget, J. Persistence of human papillomavirus DNA in benign and (pre)malignant skin lesions from renal transplant recipients. J Clin Microbiol. 2000; 38: 2087-96 [OpenAIRE] [PubMed]

Berkhout, RJ, Tieben, LM, Smits, HL, Bavinck, JN, Vermeer, BJ, ter Schegget, J. Nested PCR approach for detection and typing of epidermodysplasia verruciformis-associated human papillomavirus types in cutaneous cancers from renal transplant recipients. J Clin Microbiol. 1995; 33: 690-5 [OpenAIRE] [PubMed]

Bosch, FX, Lorincz, A, Munoz, N, Meijer, CJ, Shah, KV. The causal relation between human papillomavirus and cervical cancer. J Clin Pathol. 2002; 55: 244-65 [OpenAIRE] [PubMed]

Bouvard, V, Baan, R, Straif, K, Grosse, Y, Secretan, B, El Ghissassi, F. A review of human carcinogens--Part B: biological agents. Lancet Oncol. 2009; 10: 321-2 [PubMed]

Bouwes Bavinck, JN, Neale, RE, Abeni, D, Euvrard, S, Green, AC, Harwood, CA. Multicenter study of the association between betapapillomavirus infection and cutaneous squamous cell carcinoma. Cancer Res. 2010; 70: 9777-86 [OpenAIRE] [PubMed]

Boxman, IL, Berkhout, RJ, Mulder, LH, Wolkers, MC, Bouwes Bavinck, JN, Vermeer, BJ. Detection of human papillomavirus DNA in plucked hairs from renal transplant recipients and healthy volunteers. J Invest Dermatol. 1997; 108: 712-5 [OpenAIRE] [PubMed]

Boxman, IL, Russell, A, Mulder, LH, Bavinck, JN, ter Schegget, J, Green, A. Association between epidermodysplasia verruciformis-associated human papillomavirus DNA in plucked eyebrow hair and solar keratoses. J Invest Dermatol. 2001; 117: 1108-12 [OpenAIRE] [PubMed]

Cuypers, HT, Selten, G, Quint, W, Zijlstra, M, Maandag, ER, Boelens, W. Murine leukemia virus-induced T-cell lymphomagenesis: integration of proviruses in a distinct chromosomal region. Cell. 1984; 37: 141-50 [OpenAIRE] [PubMed]

Dang, C, Koehler, A, Forschner, T, Sehr, P, Michael, K, Pawlita, M. E6/E7 expression of human papillomavirus types in cutaneous squamous cell dysplasia and carcinoma in immunosuppressed organ transplant recipients. Br J Dermatol. 2006; 155: 129-36 [PubMed]

62 references, page 1 of 5
Related research
Abstract
β-Human papillomavirus (β-HPV) DNA is present in some cutaneous squamous cell carcinomas (cuSCCs), but no mechanism of carcinogenesis has been determined. We used ultra-high-throughput sequencing of the cancer transcriptome to assess whether papillomavirus transcripts are present in these cancers. In all, 67 cuSCC samples were assayed for β-HPV DNA by PCR, and viral loads were measured with type-specific quantitative PCR. A total of 31 SCCs were selected for whole transcriptome sequencing. Transcriptome libraries were prepared in parallel from the HPV18-positive HeLa cervical cancer cell line and HPV16-positive primary cervical and periungual SCCs. Of the tumors...
Subjects
Medical Subject Headings: virusesvirus diseases
free text keywords: Adult, Aged, and over, Betapapillomavirus, Carcinoma, Squamous Cell, Cell Transformation, Viral, DNA, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, HeLa Cells, Human papillomavirus 16, Human papillomavirus 18, Humans, Male, Middle Aged, Papillomavirus Infections, Skin Neoplasms, Uterine Cervical Neoplasms, Viral Load, Clinical Sciences, Oncology and Carcinogenesis, Dermatology & Venereal Diseases, Article, Cell Biology, Biochemistry, Molecular Biology, Dermatology, Carcinogenesis, medicine.disease_cause, medicine, Real-time polymerase chain reaction, Koilocyte, HeLa, biology.organism_classification, biology, Transcriptome, Cancer, medicine.disease
Funded by
NIH| Clinical and Translational Science Institute
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5KL2RR024130-02
  • Funding stream: NATIONAL CENTER FOR RESEARCH RESOURCES
62 references, page 1 of 5

Alam, M, Caldwell, JB, Eliezri, YD. Human papillomavirus-associated digital squamous cell carcinoma: literature review and report of 21 new cases. J Am Acad Dermatol. 2003; 48: 385-93 [PubMed]

Altschul, SF, Gish, W, Miller, W, Myers, EW, Lipman, DJ. Basic local alignment search tool. J Mol Biol. 1990; 215: 403-10 [OpenAIRE] [PubMed]

Antonsson, A, Forslund, O, Ekberg, H, Sterner, G, Hansson, BG. The ubiquity and impressive genomic diversity of human skin papillomaviruses suggest a commensalic nature of these viruses. J Virol. 2000; 74: 11636-41 [OpenAIRE] [PubMed]

Asgari, MM, Kiviat, NB, Critchlow, CW, Stern, JE, Argenyi, ZB, Raugi, GJ. Detection of human papillomavirus DNA in cutaneous squamous cell carcinoma among immunocompetent individuals. J Invest Dermatol. 2008; 128: 1409-17 [OpenAIRE] [PubMed]

Bauer, HM, Ting, Y, Greer, CE, Chambers, JC, Tashiro, CJ, Chimera, J. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA. 1991; 265: 472-7 [PubMed]

Benson, DA, Karsch-Mizrachi, I, Lipman, DJ, Ostell, J, Sayers, EW. GenBank. Nucleic Acids Res. 2009; 37: D26-31 [OpenAIRE] [PubMed]

Berkhout, RJ, Bouwes Bavinck, JN, ter Schegget, J. Persistence of human papillomavirus DNA in benign and (pre)malignant skin lesions from renal transplant recipients. J Clin Microbiol. 2000; 38: 2087-96 [OpenAIRE] [PubMed]

Berkhout, RJ, Tieben, LM, Smits, HL, Bavinck, JN, Vermeer, BJ, ter Schegget, J. Nested PCR approach for detection and typing of epidermodysplasia verruciformis-associated human papillomavirus types in cutaneous cancers from renal transplant recipients. J Clin Microbiol. 1995; 33: 690-5 [OpenAIRE] [PubMed]

Bosch, FX, Lorincz, A, Munoz, N, Meijer, CJ, Shah, KV. The causal relation between human papillomavirus and cervical cancer. J Clin Pathol. 2002; 55: 244-65 [OpenAIRE] [PubMed]

Bouvard, V, Baan, R, Straif, K, Grosse, Y, Secretan, B, El Ghissassi, F. A review of human carcinogens--Part B: biological agents. Lancet Oncol. 2009; 10: 321-2 [PubMed]

Bouwes Bavinck, JN, Neale, RE, Abeni, D, Euvrard, S, Green, AC, Harwood, CA. Multicenter study of the association between betapapillomavirus infection and cutaneous squamous cell carcinoma. Cancer Res. 2010; 70: 9777-86 [OpenAIRE] [PubMed]

Boxman, IL, Berkhout, RJ, Mulder, LH, Wolkers, MC, Bouwes Bavinck, JN, Vermeer, BJ. Detection of human papillomavirus DNA in plucked hairs from renal transplant recipients and healthy volunteers. J Invest Dermatol. 1997; 108: 712-5 [OpenAIRE] [PubMed]

Boxman, IL, Russell, A, Mulder, LH, Bavinck, JN, ter Schegget, J, Green, A. Association between epidermodysplasia verruciformis-associated human papillomavirus DNA in plucked eyebrow hair and solar keratoses. J Invest Dermatol. 2001; 117: 1108-12 [OpenAIRE] [PubMed]

Cuypers, HT, Selten, G, Quint, W, Zijlstra, M, Maandag, ER, Boelens, W. Murine leukemia virus-induced T-cell lymphomagenesis: integration of proviruses in a distinct chromosomal region. Cell. 1984; 37: 141-50 [OpenAIRE] [PubMed]

Dang, C, Koehler, A, Forschner, T, Sehr, P, Michael, K, Pawlita, M. E6/E7 expression of human papillomavirus types in cutaneous squamous cell dysplasia and carcinoma in immunosuppressed organ transplant recipients. Br J Dermatol. 2006; 155: 129-36 [PubMed]

62 references, page 1 of 5
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