publication . Other literature type . Article . 1990

Histochemical analysis of CaMV 35S promoter-β-glucuronidase gene expression in transgenic rice plants

Michael J. Battraw; Timothy C. Hall;
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  • Published: 01 Oct 1990
  • Publisher: Springer Science and Business Media LLC
Abstract
The cauliflower mosaic virus promoter is commonly used to drive transcription of chimeric genes in transgenic plants, including the cereals. To determine the tissue and cell types of cereal plants that the promoter functions in, transgenic rice plants containing a CaMV 35S promoter/GUS chimeric gene were analyzed for GUS activity. Insertion of a 35S/GUS chimeric gene at low copy number into chromosomal DNA of plants regenerated from electroporated protoplasts was confirmed by gel blot hybridization analysis of uncut and endonuclease-digested DNA. Quantitative measurement showed that GUS activity was some tenfold higher in rice leaves than in tobacco leaves [8] w...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Agronomy and Crop Science, Plant Science, Genetics, General Medicine
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