research data . Dataset . 2016

Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects

Jayendra Nath Shukla; Kalsi, Megha; Sethi, Amit; Narva, Kenneth E.; Elane Fishilevich; Singh, Satnam; Kanakachari Mogilicherla; Subba Reddy Palli;
Open Access
  • Published: 01 Jun 2016
  • Publisher: Taylor & Francis
Abstract
<p>RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We investigated the reasons for varying RNAi efficiency between lepidopteran and coleopteran cell lines and also between the Colorado potato beetle<i>, Leptinotarsa decemlineata</i> and tobacco budworm<i>, Heliothis virescens</i>. The dsRNA either injected or fed was degraded...
Subjects
Medical Subject Headings: fungi
free text keywords: Biophysics, Biochemistry, Space Science, 29999 Physical Sciences not elsewhere classified, FOS: Physical sciences, Genetics, FOS: Biological sciences, Molecular Biology, Physiology, Evolutionary Biology, 59999 Environmental Sciences not elsewhere classified, FOS: Earth and related environmental sciences, Immunology, FOS: Clinical medicine, 69999 Biological Sciences not elsewhere classified, Developmental Biology, Cancer, Plant Biology, Coleoptera, double-stranded RNA, lepidoptera, RNA interference
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Dataset . 2016
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Dataset . 2016
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Dataset . 2016
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