publication . Article . Other literature type . 2010

Crosstalk between mRNA 3′ End Processing and Transcription Initiation

edouard bertrand; Søren Lykke-Andersen; Jorgen Kjems;
Open Access
  • Published: 01 Nov 2010 Journal: Molecular Cell, volume 40, issue 3, pages 410-422 (issn: 1097-2765, Copyright policy)
  • Publisher: Elsevier BV
  • Country: France
Abstract
Summary Transcription and mRNA maturation are interdependent events. Although stimulatory connections between these processes within the same round of transcription are well described, functional coupling between separate transcription cycles remains elusive. Comparing time-resolved transcription profiles of single-copy integrated β-globin gene variants, we demonstrate that a polyadenylation site mutation decreases transcription initiation of the same gene. Upon depletion of the 3′ end processing and transcription termination factor PCF11, endogenous genes exhibit a similar phenotype. Readthrough RNA polymerase II (RNAPII) engaged on polyadenylation site-mutated...
Subjects
free text keywords: Cell Biology, Molecular Biology, Base Sequence Cyclin-Dependent Kinase 9/metabolism HEK293 Cells Humans Models, Messenger/genetics/*metabolism TATA-Box Binding Protein/metabolism Time Factors *Transcription, Biological Molecular Sequence Data Phenotype Phosphorylation Phosphoserine/metabolism Point Mutation/genetics Poly A/genetics Promoter Regions, Genetic/genetics RNA 3' End Processing/*genetics RNA Polymerase II/metabolism RNA Splicing/genetics RNA, Genetic mRNA Cleavage and Polyadenylation Factors/metabolism, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
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