publication . Article . Other literature type . 2010

Ablation of Succinate Production from Glucose Metabolism in the Procyclic Trypanosomes Induces Metabolic Switches to the Glycerol 3-Phosphate/Dihydroxyacetone Phosphate Shuttle and to Proline Metabolism

Fabien Marc Guegan; Philippe Diolez; Jean-Charles Portais;
Open Access
  • Published: 11 Aug 2010
  • Country: France
Abstract
Trypanosoma brucei is a parasitic protist that undergoes a complex life cycle during transmission from its mammalian host (bloodstream forms) to the midgut of its insect vector (procyclic form). In both parasitic forms, most glycolytic steps take place within specialized peroxisomes, called glycosomes. Here, we studied metabolic adaptations in procyclic trypanosome mutants affected in their maintenance of the glycosomal redox balance. T. brucei can theoretically use three strategies to maintain the glycosomal NAD(+)/NADH balance as follows: (i) the glycosomal succinic fermentation branch; (ii) the glycerol 3-phosphate (Gly-3-P)/dihydroxyacetone phosphate (DHAP) ...
Subjects
free text keywords: SUBSTRATE LEVEL PHOSPHORYLATION, DEPENDENT FUMARATE REDUCTASE, MOLECULAR CHARACTERIZATION, OXIDATIVE-PHOSPHORYLATION, RESPIRATORY-CHAIN, ENERGY-METABOLISM, COA-TRANSFERASE, KREBS CYCLE, INDUCIBLE EXPRESSION SYSTEM, INTRACELLULAR METABOLITES, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, SUBSTRATE LEVEL PHOSPHORYLATION;DEPENDENT FUMARATE REDUCTASE;INDUCIBLE EXPRESSION SYSTEM;MOLECULAR CHARACTERIZATION;OXIDATIVE-PHOSPHORYLATION;INTRACELLULAR METABOLITES;RESPIRATORY-CHAIN;ENERGY-METABOLISM;COA-TRANSFERASE;KREBS CYCLE, Metabolism, Cell Biology, Biochemistry, Molecular Biology
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