publication . Other literature type . Article . 2000

Patterns of Variant Polyadenylation Signal Usage in Human Genes

E Beaudoing; S Freier; J R Wyatt; J M Claverie; D Gautheret;
  • Published: 01 Jul 2000
  • Publisher: Cold Spring Harbor Laboratory
Abstract
The formation of mature mRNAs in vertebrates involves the cleavage and polyadenylation of the pre-mRNA, 10–30 nt downstream of an AAUAAA or AUUAAA signal sequence. The extensive cDNA data now available shows that these hexamers are not strictly conserved. In order to identify variant polyadenylation signals on a large scale, we compared over 8700 human 3′ untranslated sequences to 157,775 polyadenylated expressed sequence tags (ESTs), used as markers of actual mRNA 3′ ends. About 5600 EST-supported putative mRNA 3′ ends were collected and analyzed for significant hexameric sequences. Known polyadenylation signals were found in only 73% of the 3′ fragments. Ten s...
Subjects
free text keywords: Three prime untranslated region, Polyadenylation, Signal peptide, Untranslated region, Human genome, Biology, Genetics, Cleavage and polyadenylation specificity factor, Expressed sequence tag, Bioinformatics, Coding region, Letter
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publication . Other literature type . Article . 2000

Patterns of Variant Polyadenylation Signal Usage in Human Genes

E Beaudoing; S Freier; J R Wyatt; J M Claverie; D Gautheret;