publication . Other literature type . Article . 2001

Reversible Unfolding of Single RNA Molecules by Mechanical Force

Jan Liphardt; Carlos Bustamante; Bibiana Onoa; Ignacio Tinoco; Steven B. Smith;
Restricted
  • Published: 27 Apr 2001
  • Publisher: American Association for the Advancement of Science (AAAS)
Abstract
Here we use mechanical force to induce the unfolding and refolding of single RNA molecules: a simple RNA hairpin, a molecule containing a three-helix junction, and the P5abc domain of the Tetrahymena thermophila ribozyme. All three molecules (P5abc only in the absence of Mg2+) can be mechanically unfolded at equilibrium, and when kept at constant force within a critical force range, are bi-stable and hop between folded and unfolded states. We determine the force-dependent equilibrium constants for folding/unfolding these single RNA molecules and the positions of their transition states along the reaction coordinate.
doi: 10.1126/science.1058498
Subjects
free text keywords: Multidisciplinary, RNA, Tetrahymena thermophila, Equilibrium constant, Transition state, Ribozyme, biology.protein, biology, Molecule, Crystallography, Reaction coordinate, Chemistry, Mechanical force
Related Organizations
Download fromView all 2 versions
Science
Article
Provider: Microsoft Academic Graph
Science
Article . 2001
Provider: Crossref
http://dx.doi.org/10.1126/scie...
Other literature type . 2001
Provider: Datacite
American Association for the Advancement of Science (AAAS)
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue