publication . Article . Other literature type . 2007

Controlled expression of transgenes introduced byin vivoelectroporation

Matsuda, Takahiko; Cepko, Constance L.;
Open Access
  • Published: 05 Jan 2007 Journal: Proceedings of the National Academy of Sciences, volume 104, pages 1,027-1,032 (issn: 0027-8424, eissn: 1091-6490, Copyright policy)
  • Publisher: Proceedings of the National Academy of Sciences
Abstract
In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method. Here we demonstrate conditional regulation of gene expression from electroporated plasmids in the postnatal rat retina and the embryonic mouse brain. For temporal regulation, Cre/loxP-mediated inducible expression vectors were used in combination with a vector expressing a conditionally active form of Cre recombinase, which is activated by 4-hydroxytamoxifen. Onset of gene expression was regulated by the timing of 4-hydroxytamoxifen administra...
Subjects
free text keywords: Molecular biology, RNA interference, Fate mapping, Transfection, Muller glia, medicine.anatomical_structure, medicine, Regulation of gene expression, Cre recombinase, Biology, Expression vector, Electroporation, Biological Sciences
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publication . Article . Other literature type . 2007

Controlled expression of transgenes introduced byin vivoelectroporation

Matsuda, Takahiko; Cepko, Constance L.;