publication . Other literature type . Article . 2011

Malaria antifolate resistance with contrasting Plasmodium falciparum dihydrofolate reductase (DHFR) polymorphisms in humans and Anopheles mosquitoes.

Kun Liu; Sungano Mharakurwa; David J. Sullivan; Philip E. Thuma; Peter Agre; Mulenga Musapa; Mtawa A. P. Mkulama; Sandra Chishimba; Taida Kumwenda; Clive Julian Shiff;
Open Access
  • Published: 07 Nov 2011
Abstract
Surveillance for drug-resistant parasites in human blood is a major effort in malaria control. Here we report contrasting antifolate resistance polymorphisms in Plasmodium falciparum when parasites in human blood were compared with parasites in Anopheles vector mosquitoes from sleeping huts in rural Zambia. DNA encoding P. falciparum dihydrofolate reductase (EC 1.5.1.3) was amplified by PCR with allele-specific restriction enzyme digestions. Markedly prevalent pyrimethamine-resistant mutants were evident in human P. falciparum infections—S108N (>90%), with N51I, C59R, and 108N+51I+59R triple mutants (30–80%). This resistance level may be from selection pressure ...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: Biological Sciences, Multidisciplinary, Anopheles, biology.organism_classification, biology, Sulfadoxine, medicine.medical_treatment, medicine, Restriction enzyme, Pyrimethamine, medicine.drug, Virology, Malaria, medicine.disease, Dihydrofolate reductase, biology.protein, Plasmodium falciparum, Antifolate, chemistry.chemical_compound, chemistry
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Abstract
Surveillance for drug-resistant parasites in human blood is a major effort in malaria control. Here we report contrasting antifolate resistance polymorphisms in Plasmodium falciparum when parasites in human blood were compared with parasites in Anopheles vector mosquitoes from sleeping huts in rural Zambia. DNA encoding P. falciparum dihydrofolate reductase (EC 1.5.1.3) was amplified by PCR with allele-specific restriction enzyme digestions. Markedly prevalent pyrimethamine-resistant mutants were evident in human P. falciparum infections—S108N (>90%), with N51I, C59R, and 108N+51I+59R triple mutants (30–80%). This resistance level may be from selection pressure ...
Subjects
Medical Subject Headings: parasitic diseases
free text keywords: Biological Sciences, Multidisciplinary, Anopheles, biology.organism_classification, biology, Sulfadoxine, medicine.medical_treatment, medicine, Restriction enzyme, Pyrimethamine, medicine.drug, Virology, Malaria, medicine.disease, Dihydrofolate reductase, biology.protein, Plasmodium falciparum, Antifolate, chemistry.chemical_compound, chemistry
Related Organizations
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