publication . Article . Other literature type . 2007

A Sertoli Cell-Specific Knockout of Connexin43 Prevents Initiation of Spermatogenesis

Martina Zeiler; Martin Bergmann; Christina Rüttinger; Florian Guillou; Lutz Konrad; Elke Winterhager; Ralph Brehm; Katharina Biermann; Klaus Failing; Katja Herde; ...
Open Access
  • Published: 01 Jul 2007 Journal: The American Journal of Pathology, volume 171, pages 19-31 (issn: 0002-9440, Copyright policy)
  • Publisher: Elsevier BV
Abstract
The predominant testicular gap junctional protein connexin43 (cx43) is located between neighboring Sertoli cells (SCs) and between SCs and germ cells. It is assumed to be involved in testicular development, cell differentiation, initiation, and maintenance of spermatogenesis with alterations of its expression being correlated with various testicular disorders. Because total disruption of the cx43 gene leads to perinatal death, we generated a conditional cx43 knockout (KO) mouse using the Cre/loxP recombination system, which lacks the cx43 gene solely in SCs (SCCx43KO), to evaluate the SC-specific functions of cx43 on spermatogenesis in vivo. Adult SCCx43KO−/− mi...
Persistent Identifiers
Subjects
Medical Subject Headings: cardiovascular systemendocrine system
free text keywords: Pathology and Forensic Medicine, SOURIS KNOCKOUT, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], Regular Articles, Andrology, Gene, Biology, Endocrinology, medicine.medical_specialty, medicine, Testicle, medicine.anatomical_structure, Internal medicine, Epididymis, Spermatogenesis, Cellular differentiation, Mutation, medicine.disease_cause, Sertoli cell, Testicular Disorder
Abstract
The predominant testicular gap junctional protein connexin43 (cx43) is located between neighboring Sertoli cells (SCs) and between SCs and germ cells. It is assumed to be involved in testicular development, cell differentiation, initiation, and maintenance of spermatogenesis with alterations of its expression being correlated with various testicular disorders. Because total disruption of the cx43 gene leads to perinatal death, we generated a conditional cx43 knockout (KO) mouse using the Cre/loxP recombination system, which lacks the cx43 gene solely in SCs (SCCx43KO), to evaluate the SC-specific functions of cx43 on spermatogenesis in vivo. Adult SCCx43KO−/− mi...
Persistent Identifiers
Subjects
Medical Subject Headings: cardiovascular systemendocrine system
free text keywords: Pathology and Forensic Medicine, SOURIS KNOCKOUT, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], Regular Articles, Andrology, Gene, Biology, Endocrinology, medicine.medical_specialty, medicine, Testicle, medicine.anatomical_structure, Internal medicine, Epididymis, Spermatogenesis, Cellular differentiation, Mutation, medicine.disease_cause, Sertoli cell, Testicular Disorder
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