publication . Article . Other literature type . 2010

Global analysis of nascent RNA reveals transcriptional pausing in terminal exons.

Stephan Preibisch;
Open Access
  • Published: 01 Nov 2010 Journal: Molecular Cell, volume 40, pages 571-581 (issn: 1097-2765, Copyright policy)
  • Publisher: Elsevier BV
Abstract
SUMMARY Pre-mRNA splicing is catalyzed by the spliceosome, which can assemble on pre-mRNA cotranscriptionally. However, whether splicing generally occurs during transcriptionhasnotbeen addressed.Indeed, splicing catalysis is expected to occur posttranscriptionally in yeast, where the shortness of terminal exons should leave insufficient time for splicing. Here, we isolate endogenous S. cerevisiae nascent RNA and determine gene-specific splicing efficiencies and transcription profiles, using high-density tiling microarrays. Surprisingly, we find that splicing occurs cotranscriptionally for the majority of introncontaining genes. Analysis of transcription profiles...
Subjects
free text keywords: Cell Biology, Molecular Biology
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