publication . Other literature type . Article . 1975

The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors

A P Halestrap;
  • Published: 01 Apr 1975
  • Publisher: Portland Press Ltd.
Abstract
1. Studies on the kinetics of pyruvate transport into mitochondria by an 'inhibitor-stop' technique were hampered by the decarboxylation of pyruvate by mitochondria even in the presence of rotenone. Decarboxylation was minimal at 6 degrees C. At this temperature the Km for pyruvate was 0.15 mM and Vmax. was 0.54nmol/min per mg of protein; alpha-cyano-4-hydroxycinnamate was found to be a non-competitive inhibitor, Ki 6.3 muM, and phenyl-pyruvate a competitive inhibitor, Ki 1.8 mM. 2. At 100 muM concentration, alpha-cyano-4-hydroxycinnamate rapidly and almost totally inhibited O2 uptake by rat heart mitochondria oxidizing pyruvate. Inhibition could be detected at ...
Subjects
free text keywords: Research Article, Cell Biology, Biochemistry, Molecular Biology, Mitochondrion, Mitochondrial pyruvate transport, Pyruvate dehydrogenase kinase, Pyruvate dehydrogenase complex, Pyruvate carboxylase, Chemistry, Pyruvate decarboxylation, Pyruvate transport, Gluconeogenesis
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publication . Other literature type . Article . 1975

The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors

A P Halestrap;