publication . Article . Other literature type . 2009

Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei

Peter Myler; Marilyn Parsons;
Open Access English
  • Published: 01 Oct 2009 Journal: BMC Genomics, volume 10, pages 482-482 (eissn: 1471-2164, Copyright policy)
  • Publisher: BioMed Central
Abstract
<p>Abstract</p> <p>Background</p> <p><it>Trypanosoma brucei</it>, the causative agent of African sleeping sickness, undergoes a complex developmental cycle that takes place in mammalian and insect hosts and is accompanied by changes in metabolism and cellular morphology. While differences in mRNA expression have been described for many genes, genome-wide expression analyses have been largely lacking. Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation.</p> <p>Results</p> <p>Here we present a comprehensive analy...
Subjects
free text keywords: Research Article, Biotechnology, Genetics, TP248.13-248.65, QH426-470
Funded by
NIH| Transcription of protein-coding genes in Leishmania
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01AI053667-03
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
,
NIH| TYROSINE KINASE OF TRYPANOSOMA BRUCEI
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01AI031077-03
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
61 references, page 1 of 5

Taylor, JE, Rudenko, G. Switching trypanosome coats: what's in the wardrobe?. Trends Genet. 2006; 22: 614-620 [OpenAIRE] [PubMed] [DOI]

Vickerman, K. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature. 1965; 208: 762-766 [OpenAIRE] [PubMed] [DOI]

Flynn, IW, Bowman, IBR. The metabolism of carbohydrate by pleomorphic African trypanosomes. Comp Biochem Physiol. 1973; 45B: 25-42

Bienen, EJ, Saric, M, Pollakis, G, Grady, RW, Clarkson, AB. Mitochondrial development in Trypanosoma brucei brucei transitional bloodstream forms. Mol Biochem Parasitol. 1991; 45: 185-192 [OpenAIRE] [PubMed] [DOI]

Vassella, E, Acosta-Serrano, A, Studer, E, Lee, SH, Englund, PT, Roditi, I. Multiple procyclin isoforms are expressed differentially during the development of insect forms of Trypanosoma brucei. J Mol Biol. 2001; 312: 597-607 [OpenAIRE] [PubMed] [DOI]

Vickerman, K. Developmental cycles and biology of pathogenic trypanosomes. Brit Med Bull. 1985; 41: 105-114 [OpenAIRE] [PubMed]

Clayton, CE. Life without transcriptional control? From fly to man and back again. EMBO J. 2002; 21: 1881-1888 [OpenAIRE] [PubMed] [DOI]

Clayton, C, Shapira, M. Post-transcriptional regulation of gene expression in trypanosomes and leishmanias. Mol Biochem Parasitol. 2007; 156: 93-101 [OpenAIRE] [PubMed] [DOI]

Diehl, S, Diehl, F, El Sayed, NM, Clayton, C, Hoheisel, JD. Analysis of stage-specific gene expression in the bloodstream and the procyclic form of Trypanosoma brucei using a genomic DNA-microarray. Mol Biochem Parasitol. 2002; 123: 115-123 [PubMed] [DOI]

Brems, S, Lys, GD, Gundlesdodjir-Planck, D, Busold, C, Luu, VD, Schanne, M, Hoheisel, J, Clayton, C. The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes. Mol Biochem Parasitol. 2005; 139: 163-172 [OpenAIRE] [PubMed] [DOI]

Berriman, M, Ghedin, E, Hertz-Fowler, C, Blandin, G, Renauld, H, Bartholomeu, DC, Lennard, NJ, Caler, E, Hamlin, NE, Haas, B, Bohme, U, Hannick, L, Aslett, MA, Shallom, J, Marcello, L, Hou, L, Wickstead, B, Alsmark, UC, Arrowsmith, C, Atkin, RJ, Barron, AJ, Bringaud, F, Brooks, K, Carrington, M, Cherevach, I, Chillingworth, TJ, Churcher, C, Clark, LN, Corton, CH, Cronin, A, Davies, RM, Doggett, J, Djikeng, A, Feldblyum, T, Field, MC, Fraser, A, Goodhead, I, Hance, Z, Harper, D, Harris, BR, Hauser, H, Hostetler, J, Ivens, A, Jagels, K, Johnson, D, Johnson, J, Jones, K, Kerhornou, AX, Koo, H, Larke, N, Landfear, S, Larkin, C, Leech, V, Line, A, Lord, A, MacLeod, A, Mooney, PJ, Moule, S, Martin, DM, Morgan, GW, Mungall, K, Norbertczak, H, Ormond, D, Pai, G, Peacock, CS, Peterson, J, Quail, MA, Rabbinowitsch, E, Rajandream, MA, Reitter, C, Salzberg, SL, Sanders, M, Schobel, S, Sharp, S, Simmonds, M, Simpson, AJ, Tallon, L, Turner, CM, Tait, A, Tivey, AR, Van Aken, S, Walker, D, Wanless, D, Wang, S, White, B, White, O, Whitehead, S, Woodward, J, Wortman, J, Adams, MD, Embley, TM, Gull, K, Ullu, E, Barry, JD, Fairlamb, AH, Opperdoes, F, Barrell, BG, Donelson, JE, Hall, N, Fraser, CM, Melville, SE, El Sayed, NM. The genome of the African trypanosome Trypanosoma brucei. Science. 2005; 309: 416-422 [OpenAIRE] [PubMed] [DOI]

Koumandou, VL, Natesan, SK, Sergeenko, T, Field, MC. The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages. BMC Genomics. 2008; 9: 298 [OpenAIRE] [PubMed] [DOI]

Hartmann, C, Benz, C, Brems, S, Ellis, L, Luu, VD, Stewart, M, D'Orso, I, Busold, C, Fellenberg, K, Frasch, AC, Carrington, M, Hoheisel, J, Clayton, CE. The small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes. Eukaryot Cell. 2007; 6: 1964-1978 [OpenAIRE] [PubMed] [DOI]

Stern, MZ, Gupta, SK, Salmon-Divon, M, Haham, T, Barda, O, Levi, S, Wachtel, C, Nilsen, TW, Michaeli, S. Multiple roles for polypyrimidine tract binding (PTB) proteins in trypanosome RNA metabolism. RNA. 2009; 15: 648-665 [OpenAIRE] [PubMed] [DOI]

Thomas, S, Green, A, Sturm, NR, Campbell, DA, Myler, PJ. Histone acetylations mark origins of polycistronic transcription in Leishmania major. BMC Genomics. 2009; 10: 152 [OpenAIRE] [PubMed] [DOI]

61 references, page 1 of 5
Related research
Abstract
<p>Abstract</p> <p>Background</p> <p><it>Trypanosoma brucei</it>, the causative agent of African sleeping sickness, undergoes a complex developmental cycle that takes place in mammalian and insect hosts and is accompanied by changes in metabolism and cellular morphology. While differences in mRNA expression have been described for many genes, genome-wide expression analyses have been largely lacking. Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation.</p> <p>Results</p> <p>Here we present a comprehensive analy...
Subjects
free text keywords: Research Article, Biotechnology, Genetics, TP248.13-248.65, QH426-470
Funded by
NIH| Transcription of protein-coding genes in Leishmania
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01AI053667-03
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
,
NIH| TYROSINE KINASE OF TRYPANOSOMA BRUCEI
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01AI031077-03
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
61 references, page 1 of 5

Taylor, JE, Rudenko, G. Switching trypanosome coats: what's in the wardrobe?. Trends Genet. 2006; 22: 614-620 [OpenAIRE] [PubMed] [DOI]

Vickerman, K. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature. 1965; 208: 762-766 [OpenAIRE] [PubMed] [DOI]

Flynn, IW, Bowman, IBR. The metabolism of carbohydrate by pleomorphic African trypanosomes. Comp Biochem Physiol. 1973; 45B: 25-42

Bienen, EJ, Saric, M, Pollakis, G, Grady, RW, Clarkson, AB. Mitochondrial development in Trypanosoma brucei brucei transitional bloodstream forms. Mol Biochem Parasitol. 1991; 45: 185-192 [OpenAIRE] [PubMed] [DOI]

Vassella, E, Acosta-Serrano, A, Studer, E, Lee, SH, Englund, PT, Roditi, I. Multiple procyclin isoforms are expressed differentially during the development of insect forms of Trypanosoma brucei. J Mol Biol. 2001; 312: 597-607 [OpenAIRE] [PubMed] [DOI]

Vickerman, K. Developmental cycles and biology of pathogenic trypanosomes. Brit Med Bull. 1985; 41: 105-114 [OpenAIRE] [PubMed]

Clayton, CE. Life without transcriptional control? From fly to man and back again. EMBO J. 2002; 21: 1881-1888 [OpenAIRE] [PubMed] [DOI]

Clayton, C, Shapira, M. Post-transcriptional regulation of gene expression in trypanosomes and leishmanias. Mol Biochem Parasitol. 2007; 156: 93-101 [OpenAIRE] [PubMed] [DOI]

Diehl, S, Diehl, F, El Sayed, NM, Clayton, C, Hoheisel, JD. Analysis of stage-specific gene expression in the bloodstream and the procyclic form of Trypanosoma brucei using a genomic DNA-microarray. Mol Biochem Parasitol. 2002; 123: 115-123 [PubMed] [DOI]

Brems, S, Lys, GD, Gundlesdodjir-Planck, D, Busold, C, Luu, VD, Schanne, M, Hoheisel, J, Clayton, C. The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes. Mol Biochem Parasitol. 2005; 139: 163-172 [OpenAIRE] [PubMed] [DOI]

Berriman, M, Ghedin, E, Hertz-Fowler, C, Blandin, G, Renauld, H, Bartholomeu, DC, Lennard, NJ, Caler, E, Hamlin, NE, Haas, B, Bohme, U, Hannick, L, Aslett, MA, Shallom, J, Marcello, L, Hou, L, Wickstead, B, Alsmark, UC, Arrowsmith, C, Atkin, RJ, Barron, AJ, Bringaud, F, Brooks, K, Carrington, M, Cherevach, I, Chillingworth, TJ, Churcher, C, Clark, LN, Corton, CH, Cronin, A, Davies, RM, Doggett, J, Djikeng, A, Feldblyum, T, Field, MC, Fraser, A, Goodhead, I, Hance, Z, Harper, D, Harris, BR, Hauser, H, Hostetler, J, Ivens, A, Jagels, K, Johnson, D, Johnson, J, Jones, K, Kerhornou, AX, Koo, H, Larke, N, Landfear, S, Larkin, C, Leech, V, Line, A, Lord, A, MacLeod, A, Mooney, PJ, Moule, S, Martin, DM, Morgan, GW, Mungall, K, Norbertczak, H, Ormond, D, Pai, G, Peacock, CS, Peterson, J, Quail, MA, Rabbinowitsch, E, Rajandream, MA, Reitter, C, Salzberg, SL, Sanders, M, Schobel, S, Sharp, S, Simmonds, M, Simpson, AJ, Tallon, L, Turner, CM, Tait, A, Tivey, AR, Van Aken, S, Walker, D, Wanless, D, Wang, S, White, B, White, O, Whitehead, S, Woodward, J, Wortman, J, Adams, MD, Embley, TM, Gull, K, Ullu, E, Barry, JD, Fairlamb, AH, Opperdoes, F, Barrell, BG, Donelson, JE, Hall, N, Fraser, CM, Melville, SE, El Sayed, NM. The genome of the African trypanosome Trypanosoma brucei. Science. 2005; 309: 416-422 [OpenAIRE] [PubMed] [DOI]

Koumandou, VL, Natesan, SK, Sergeenko, T, Field, MC. The trypanosome transcriptome is remodelled during differentiation but displays limited responsiveness within life stages. BMC Genomics. 2008; 9: 298 [OpenAIRE] [PubMed] [DOI]

Hartmann, C, Benz, C, Brems, S, Ellis, L, Luu, VD, Stewart, M, D'Orso, I, Busold, C, Fellenberg, K, Frasch, AC, Carrington, M, Hoheisel, J, Clayton, CE. The small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes. Eukaryot Cell. 2007; 6: 1964-1978 [OpenAIRE] [PubMed] [DOI]

Stern, MZ, Gupta, SK, Salmon-Divon, M, Haham, T, Barda, O, Levi, S, Wachtel, C, Nilsen, TW, Michaeli, S. Multiple roles for polypyrimidine tract binding (PTB) proteins in trypanosome RNA metabolism. RNA. 2009; 15: 648-665 [OpenAIRE] [PubMed] [DOI]

Thomas, S, Green, A, Sturm, NR, Campbell, DA, Myler, PJ. Histone acetylations mark origins of polycistronic transcription in Leishmania major. BMC Genomics. 2009; 10: 152 [OpenAIRE] [PubMed] [DOI]

61 references, page 1 of 5
Related research
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue