publication . Article . 2009

Molecular and phenotypic characterization of invasive group B streptococcus strains from infants in Norway 2006–2007

Håkon Bergseng; Jan Egil Afset; Andreas Radtke; K. Loeseth; Randi Valsø Lyng; Marite Rygg; Kåre Bergh;
Open Access
  • Published: 18 May 2009 Journal: Clinical Microbiology and Infection, volume 15, pages 1,182-1,185 (issn: 1198-743X, Copyright policy)
  • Publisher: Elsevier BV
Abstract
AbstractMultilocus sequence typing of an almost complete collection of invasive group B streptococcus (GBS) strains from infants in Norway, conducted in 2006–2007, revealed 27 sequence types (ST), of which 23 clustered into five clonal complexes. The case fatality rate of invasive GBS disease in infants was 16/98 (16.3%). Type V strains were predominant among strains resistant to erythromycin and clindamycin (11/18; 61.1%). All type V strains from fatal cases (5/16) were ST1, resistant to erythromycin and clindamycin, and belonged to three pulsed-field gel electrophoresis-clusters. Further analysis of virulence characteristics of these apparently highly virulent...
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Subjects
free text keywords: Microbiology (medical), Infectious Diseases, General Medicine, Antibiotic susceptibility, early onset disease, invasive GBS disease, Typing, Pulsed-field gel electrophoresis, Virulence, Streptococcus, medicine.disease_cause, medicine, Biology, Multilocus sequence typing, Microbiology, Clindamycin, medicine.drug, Erythromycin, Antibacterial agent

1. Jones N, Bohnsack JF, Takahashi S et al. Multilocus sequence typing system for group B streptococcus. J Clin Microbiol 2003; 41: 2530- 2536.

2. Feil EJ, Li BC, Aanensen DM et al. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 2004; 186: 1518-30.

3. Zeng X, Kong F, Morgan J et al. Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus agalactiae. J Clin Microbiol 2006; 44: 3822-3825.

4. Creti R, Fabretti F, Orefici G et al. Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes. J Clin Microbiol 2004; 42: 1326-1329.

5. Kong F, Gowan S, Martin D et al. Serotype identification of group B streptococci by PCR and sequencing. J Clin Microbiol 2002; 40: 216-226.

6. Skjaervold NK, Bergh K, Bevanger L. Distribution of PFGE types of invasive Norwegian group B streptococci in relation to serotypes. Indian J Med Res 2004; 119 (suppl): 201-204.

7. Bisharat N, Jones N, Marchaim D et al. Population structure of group B streptococcus from a low-incidence region for invasive neonatal disease. Microbiology 2005; 151:1875-1881.

8. Luan SL, Granlund M, Sellin M et al. Multilocus sequence typing of Swedish invasive group B streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching. J Clin Microbiol 2005; 43: 3727-3733.

9. Bohnsack JF, Whiting A, Gottschalk M et al. Population structure of invasive and colonizing strains of Streptococcus agalactiae from neonates of six US Academic Centers from 1995 to 1999. J Clin Microbiol 2008; 46: 1285-1291.

10. Sun Y, Kong F, Zhao Z et al. Comparison of a 3-set genotyping system with multilocus sequence typing for Streptococcus agalactiae (group B streptococcus). J Clin Microbiol 2005; 43: 4704-4707.

11. Zhao Z, Kong F, Zeng X et al. Distribution of genotypes and antibiotic resistance genes among invasive Streptococcus agalactiae (group B streptococcus) isolates from Australasian patients belonging to different age groups. Clin Microbiol Infect 2008; 14: 260-267. [OpenAIRE]

12. Bergseng H, Rygg M, Bevanger L et al. Invasive group B streptococcus (GBS) disease in Norway 1996-2006. Eur J Clin Microbiol Infect Dis 2008; 27: 1193-1199.

13. Diekema DJ, Andrews JI, Huynh H et al. Molecular epidemiology of macrolide resistance in neonatal bloodstream isolates of group B streptococci. J Clin Microbiol 2003; 41: 2659-2661.

14. Elliott JA, Farmer KD, Facklam RR. Sudden increase in isolation of group B streptococci, serotype V, is not due to emergence of a new pulsedfield gel electrophoresis type. J Clin Microbiol 1998; 36: 2115-2116. [OpenAIRE]

15. von Both U, Ruess M, Mueller U et al. A serotype V clone is predominant among erythromycin-resistant Streptococcus agalactiae isolates in a southwestern region of Germany. J Clin Microbiol 2003; 41: 2166-9.

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