publication . Other literature type . Article . 1983

A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS

Blakeney, A. B.; Harris, P. J.; Robert Henry; Stone, B. A.;
Restricted
  • Published: 01 Mar 1983
  • Publisher: Elsevier BV
Abstract
Abstract A simple and rapid method is described for the preparation of alditol acetates from monosaccharides. It can be performed in a single tube without transfers or evaporations. Monosaccharides are reduced with sodium borohydride in dimethyl sulphoxide and the resulting alditols acetylated using 1-methylimidazole as the catalyst. Removal of borate is unnecessary and acetylation is complete in 10 min at room temperature. Monosaccharides are quantitatively reduced and acetylated by this procedure. The alditol acetates are completely separated by glass-capillary, gas-liquid chromatography on Silar 10C. The method has been applied to the analysis of monosacchari...
Subjects
free text keywords: Organic Chemistry, Analytical Chemistry, Biochemistry, General Medicine
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue