research data . Dataset . 2019

The GID ubiquitin ligase complex is a regulator of AMPK activity and organismal lifespan

Huaize Liu; Ding, Jie; Köhnlein, Karl; Urban, Nadine; Ori, Alessandro; Villavicencio-Lorini, Pablo; Walentek, Peter; Lars-Oliver Klotz; Hollemann, Thomas; Pfirrmann, Thorsten;
Open Access
  • Published: 04 Dec 2019
  • Publisher: Taylor & Francis
Abstract
<p>The AMP-activated protein kinase (AMPK) regulates cellular energy homeostasis by sensing the metabolic status of the cell. AMPK is regulated by phosphorylation and dephosphorylation as a result of changing AMP/ATP levels and by removal of inhibitory ubiquitin residues by USP10. In this context, we identified the GID-complex, an evolutionarily conserved ubiquitin-ligase-complex (E3), as a negative regulator of AMPK activity. Our data show that the GID-complex targets AMPK for ubiquitination thereby altering its activity. Cells depleted of GID-subunits mimic a state of starvation as shown by increased AMPK activity and macroautophagic/autophagic flux as well as...
Subjects
free text keywords: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, 39999 Chemical Sciences not elsewhere classified, Science Policy, Immunology, FOS: Biological sciences, FOS: Chemical sciences, FOS: Clinical medicine, AMPK, autophagy, GID, longevity, primary cilium, ubiquitination
Communities
Science and Innovation Policy Studies
Download fromView all 6 versions
figshare
Dataset . 2019
Provider: Datacite
figshare
Dataset . 2019
Provider: Datacite
figshare
Dataset . 2019
Provider: figshare
Abstract
<p>The AMP-activated protein kinase (AMPK) regulates cellular energy homeostasis by sensing the metabolic status of the cell. AMPK is regulated by phosphorylation and dephosphorylation as a result of changing AMP/ATP levels and by removal of inhibitory ubiquitin residues by USP10. In this context, we identified the GID-complex, an evolutionarily conserved ubiquitin-ligase-complex (E3), as a negative regulator of AMPK activity. Our data show that the GID-complex targets AMPK for ubiquitination thereby altering its activity. Cells depleted of GID-subunits mimic a state of starvation as shown by increased AMPK activity and macroautophagic/autophagic flux as well as...
Subjects
free text keywords: Biophysics, Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Physiology, Biotechnology, 39999 Chemical Sciences not elsewhere classified, Science Policy, Immunology, FOS: Biological sciences, FOS: Chemical sciences, FOS: Clinical medicine, AMPK, autophagy, GID, longevity, primary cilium, ubiquitination
Communities
Science and Innovation Policy Studies
Download fromView all 6 versions
figshare
Dataset . 2019
Provider: Datacite
figshare
Dataset . 2019
Provider: Datacite
figshare
Dataset . 2019
Provider: figshare
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