publication . Article . Other literature type . 1989

Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein.

Ge, A Z; Shivarova, N I; Dean, D H;
  • Published: 01 Jun 1989
Abstract
Abstract Bacillus thuringiensis produces different types of insecticidal crystal proteins (ICPs) or delta-endotoxins. In an effort to identify the insect specificity of ICP toxins, two icp genes were cloned into the Escherichia coli expression vector pKK223-3, and bioassays were performed with purified crystals. The type A protein [from an icpA1, or 4.5-kilobase (kb) gene, from B. thuringiensis var. kurstaki HD-1] was found to be 400 times more active against Bombyx mori than type C protein (from an icpC73, or 6.6-kb gene, from B. thuringiensis var. kurstaki HD-244). The type C protein was 9 times more active against Trichoplusia ni than the type A protein, whil...
Subjects
Medical Subject Headings: fungi
free text keywords: Multidisciplinary, Bacillus thuringiensis, biology.organism_classification, biology, Site-directed mutagenesis, Molecular cloning, Molecular biology, Bombyx mori, Delta endotoxin, Expression vector, Bombycidae, Bombyx, Research Article
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