publication . Article . Other literature type . 2011

Mycolactone impairs T cell homing by suppressing microRNA control of L-selectin expression

Caroline Demangel;
Open Access English
  • Published: 02 Aug 2011 Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (issn: 0027-8424, Copyright policy)
  • Publisher: NATL ACAD SCIENCES
  • Country: France
Abstract
International audience; Mycolactone is a macrolide produced by Mycobacterium ulcerans with immunomodulatory properties. Here, we describe that in mouse, mycolactone injection led to a massive T-cell depletion in peripheral lymph nodes (PLNs) that was associated with defective expression of L-selectin (CD62-L). Importantly, preexposure to mycolactone impaired the capacity of T cells to reach PLNs after adoptive transfer, respond to chemotactic signals, and expand upon antigenic stimulation in vivo. We found that mycolactone-induced suppression of CD62-L expression by human primary T cells was induced rapidly at both the mRNA and protein levels and correlated with...
Subjects
free text keywords: MESH: Animals, MESH: Bacterial Toxins, MESH: Gene Expression Profiling, MESH: Humans, MESH: Jurkat Cells, MESH: L-Selectin, MESH: Lymph Nodes, MESH: Mice, MESH: Mice, Inbred C57BL, MESH: MicroRNAs, MESH: Oligonucleotide Array Sequence Analysis, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: CD4-Positive T-Lymphocytes, MESH: T-Lymphocytes, MESH: Time Factors, MESH: CD8-Positive T-Lymphocytes, MESH: Cell Movement, MESH: Cells, Cultured, MESH: Dose-Response Relationship, Drug, MESH: Female, MESH: Flow Cytometry, MESH: Gene Expression, [SDV.IMM]Life Sciences [q-bio]/Immunology, Biological Sciences, MESH : Animals, MESH : Bacterial Toxins, MESH : Gene Expression Profiling, MESH : Humans, MESH : Jurkat Cells, MESH : L-Selectin, MESH : Lymph Nodes, MESH : Mice, MESH : Mice, Inbred C57BL, MESH : MicroRNAs, MESH : Oligonucleotide Array Sequence Analysis, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH : CD4-Positive T-Lymphocytes, MESH : T-Lymphocytes, MESH : Time Factors, MESH : CD8-Positive T-Lymphocytes, MESH : Cell Movement, MESH : Cells, Cultured, MESH : Dose-Response Relationship, Drug, MESH : Female, MESH : Flow Cytometry, MESH : Gene Expression, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Multidisciplinary
Funded by
EC| BURULIVAC
Project
BURULIVAC
Identification and development of vaccine candidates for Buruli Ulcer Disease
  • Funder: European Commission (EC)
  • Project Code: 241500
  • Funding stream: FP7 | SP1 | HEALTH
Abstract
International audience; Mycolactone is a macrolide produced by Mycobacterium ulcerans with immunomodulatory properties. Here, we describe that in mouse, mycolactone injection led to a massive T-cell depletion in peripheral lymph nodes (PLNs) that was associated with defective expression of L-selectin (CD62-L). Importantly, preexposure to mycolactone impaired the capacity of T cells to reach PLNs after adoptive transfer, respond to chemotactic signals, and expand upon antigenic stimulation in vivo. We found that mycolactone-induced suppression of CD62-L expression by human primary T cells was induced rapidly at both the mRNA and protein levels and correlated with...
Subjects
free text keywords: MESH: Animals, MESH: Bacterial Toxins, MESH: Gene Expression Profiling, MESH: Humans, MESH: Jurkat Cells, MESH: L-Selectin, MESH: Lymph Nodes, MESH: Mice, MESH: Mice, Inbred C57BL, MESH: MicroRNAs, MESH: Oligonucleotide Array Sequence Analysis, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH: CD4-Positive T-Lymphocytes, MESH: T-Lymphocytes, MESH: Time Factors, MESH: CD8-Positive T-Lymphocytes, MESH: Cell Movement, MESH: Cells, Cultured, MESH: Dose-Response Relationship, Drug, MESH: Female, MESH: Flow Cytometry, MESH: Gene Expression, [SDV.IMM]Life Sciences [q-bio]/Immunology, Biological Sciences, MESH : Animals, MESH : Bacterial Toxins, MESH : Gene Expression Profiling, MESH : Humans, MESH : Jurkat Cells, MESH : L-Selectin, MESH : Lymph Nodes, MESH : Mice, MESH : Mice, Inbred C57BL, MESH : MicroRNAs, MESH : Oligonucleotide Array Sequence Analysis, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH : CD4-Positive T-Lymphocytes, MESH : T-Lymphocytes, MESH : Time Factors, MESH : CD8-Positive T-Lymphocytes, MESH : Cell Movement, MESH : Cells, Cultured, MESH : Dose-Response Relationship, Drug, MESH : Female, MESH : Flow Cytometry, MESH : Gene Expression, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Multidisciplinary
Funded by
EC| BURULIVAC
Project
BURULIVAC
Identification and development of vaccine candidates for Buruli Ulcer Disease
  • Funder: European Commission (EC)
  • Project Code: 241500
  • Funding stream: FP7 | SP1 | HEALTH
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