publication . Other literature type . Article . 1994

Sensing Discrete Streptavidin-Biotin Interactions with Atomic Force Microscopy

Richard J. Colton; Gil U. Lee; David A. Kidwell;
  • Published: 01 Feb 1994
  • Publisher: American Chemical Society (ACS)
Molecular recognition forms the basis for assembly and regulation in living organisms. We have used the atomic force microscope (AFM) to study the interaction of a model receptor streptavidin with its ligand biotin under physiological conditions. Surfaces functionalized with biotin and streptavidin exhibited adhesive forces 3-8 times greater than the nonspecific interactions observed between blocked streptavidin and biotinylated surfaces. The magnitude and distribution of the observed adhesive forces suggest they result from individual streptavidin-biotin interactions. This technique provides a means to directly study molecular recognition interactions at the mo...
free text keywords: Spectroscopy, Electrochemistry, General Materials Science, Surfaces and Interfaces, Condensed Matter Physics, Molecular recognition, Nanotechnology, Chemistry, Biotinylation, Streptavidin, chemistry.chemical_compound, Intermolecular force, Folding (chemistry), Supramolecular chemistry, Surface forces apparatus, Biophysics, Non-covalent interactions, chemistry.chemical_classification
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