publication . Article . Other literature type . 1994

In situ localization of the hepatocytic Na+/Taurocholate cotransporting polypeptide in rat liver

Stieger, Bruno; Hagenbuch, Bruno; Landmann, Lukas; Höchli, Mathias; Schroeder, Alice; Meier, Peter J.;
  • Published: 01 Dec 1994 Journal: Gastroenterology, volume 107, pages 1,781-1,787 (issn: 0016-5085, Copyright policy)
  • Publisher: Elsevier BV
BACKGROUND/AIMS: An Na+/taurocholate cotransporting polypeptide (Ntcp) has recently been cloned from rat liver. The aim of this study was to directly characterize the native Ntcp on the protein level and study its in situ distribution in rat liver. METHODS: A rabbit antiserum was raised against a fusion protein containing the maltose-binding protein and the C terminus of Ntcp. Native Ntcp was localized in situ by immunofluorescent techniques. Expression of Ntcp was directly correlated with taurocholate uptake measurements in stably transfected Chinese hamster ovary cells. RESULTS: Native Ntcp showed an apparent molecular weight of 51,000. After deglycosylation o...
Medical Subject Headings: digestive system
free text keywords: Gastroenterology
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