publication . Article . 2012

Over a century of neuron culture: From the hanging drop to microfluidic devices

Larry Millet; Gillette, M. U.;
Open Access
  • Published: 01 Dec 2012
Abstract
The brain is the most intricate, energetically active, and plastic organ in the body. These features extend to its cellular elements, the neurons and glia. Understanding neurons, or nerve cells, at the cellular and molecular levels is the cornerstone of modern neuroscience. The complexities of neuron structure and function require unusual methods of culture to determine how aberrations in or between cells give rise to brain dysfunction and disease. Here we review the methods that have emerged over the past century for culturing neurons in vitro, from the landmark finding by Harrison (1910) — that neurons can be cultured outside the body — to studies utilizing cu...
Subjects
Medical Subject Headings: nervous system
free text keywords: Focus: Neuroscience, brain slice culture, cell culture, co-culture, culture chamber, culture flask, culture substrate, hanging drop, organotypic culture, microfluidics, neuron, polydimethylsiloxane, PDMS, substrate patterning
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