publication . Article . Other literature type . 1991

Measurement of de novo hepatic lipogenesis in humans using stable isotopes.

Kathleen Mulligan; S. Kaempfer; Cedric H.L. Shackleton; M. Christiansen; J. S. Reid; N. S. Hellerstein; Marc K. Hellerstein; K. Wu; C. Kletke;
Open Access
  • Published: 01 May 1991 Journal: Journal of Clinical Investigation, volume 87, pages 1,841-1,852 (issn: 0021-9738, Copyright policy)
  • Publisher: American Society for Clinical Investigation
Abstract
Direct measurement of de novo lipogenesis has not previously been possible in humans. We measured de novo hepatic lipogenesis in normal men by means of stable isotopes and by combining the acetylated-xenobiotic probe technique with mass isotopomer analysis of secreted very low density lipoprotein-fatty acids (VLDL-FA). Sulfamethoxazole (SMX) was administered with [13C]acetate during an overnight fast followed by refeeding with intravenous glucose (7-10 mg/kg of weight per min), oral Ensure (7-10 mg of carbohydrate/kg of weight per min), or a high-carbohydrate mixed-meal breakfast (3.5 g of carbohydrate/kg of weight). Respiratory quotients remained less than 1.0....
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free text keywords: General Medicine, Research Article, Stearate, chemistry.chemical_compound, chemistry, Carbohydrate, Isotopes of carbon, Dilution, Lipogenesis, Fatty acid, chemistry.chemical_classification, Chromatography, Stable isotope ratio, Mole
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