publication . Other literature type . Article . 2007

Identification of a trypsin-like serine protease from Trichoderma reesei QM9414

Folke Tjerneld; Per Hägglund; Dóra Dienes; Henrik Stålbrand; Gunnar Lidén; Kati Réczey; Johan Börjesson;
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  • Published: 01 Apr 2007
  • Publisher: Elsevier BV
Abstract
Abstract In the present work the genetically modified Trichoderma reesei strain QM9414 was used to produce full-length Cel7B (endoglucanase I, EGI) under the control of the constitutive Aspergillus nidulans gpdA promoter in the presence of glucose. However, the full-length Cel7B enzyme was found to be truncated to lower molecular weight components in the culture broth. Truncation of recombinant proteins produced in fungi may be due to protease activity. In order to identify major sectreted proteases, protease activity was assessed in culture filtrate of the T. reesei QM9414 recombinant. Zymogram analysis revealed the presence of proteolytic activity correspondin...
Subjects
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, Biochemistry, Trichoderma reesei, biology.organism_classification, biology, Molecular mass, Protease, medicine.medical_treatment, medicine, Trypsin, medicine.drug, Isoelectric point, Serine protease, biology.protein, Proteases, Fungal protein
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