publication . Article . Other literature type . 1996

Stabilization of Triple-Stranded Oligonucleotide Complexes: Use of Probes Containing Alternating Phosphodiester and Stereo-Uniform Cationic Phosphoramidate Linkages

Thomas Horn; Robert L. Letsinger; Surendra Chaturvedi;
Open Access
  • Published: 15 Jun 1996 Journal: Nucleic Acids Research, volume 24, pages 2,318-2,323 (issn: 0305-1048, eissn: 1362-4962, Copyright policy)
  • Publisher: Oxford University Press (OUP)
Abstract
Pyrimidine oligonucleotides containing alternating anionic and stereo-uniform cationic N-(dimethylamino-propyl)phosphoramidate linkages [e.g. d(T+T-)7T, d(T+T-)2(T+C-)5T and (U'+U'-)7dT, where U' is 2'-O-methyluridine)] are shown to bind to complementary double-stranded DNA segments in 0.1 M NaCl at pH 7 to form triple-stranded complexes with the pyrimidine.purine.pyrimidine motif. For each of the sequences investigated, one stereoisomer bound with higher affinity, and the other stereoisomer with lower affinity, than the corresponding all-phosphodiester oligonucleotide. The stereoisomer of d(T+T-)7T that interacted weakly with a dT.dA target in 0.1 M NaCl formed...
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Subjects
free text keywords: Research Article, Stereochemistry, Thymidine, chemistry.chemical_compound, chemistry, Pyrimidine, Oligonucleotide, Purine metabolism, Cationic polymerization, DNA, Biology, Phosphodiester bond, Biochemistry, Phosphoramidate
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