publication . Article . Other literature type . 2008

Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs

Yangming null;
Open Access
  • Published: 15 Oct 2008 Journal: Genes & Development, volume 22, pages 2,773-2,785 (issn: 0890-9369, Copyright policy)
  • Publisher: Cold Spring Harbor Laboratory
Abstract
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8Δ/Δ mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1Δ/Δ mESCs, suggesting a physiological role for Microprocessor-independent, Dicer-dependent small RNAs. To identify these small RNAs with unusual biogenesis, we performed high-throughput sequencing from wild-type, dgcr8Δ/Δ, and dicer1Δ/Δ mESCs. Several of the resulting DGCR8-independent, Dicer-dependent RNAs were noncanonical miRNAs. These derived from mirtrons and a newly identified subclass of miRNA precursors, w...
Subjects
free text keywords: Developmental Biology, Genetics, Research Paper
Abstract
Canonical microRNAs (miRNAs) require two processing steps: the first by the Microprocessor, a complex of DGCR8 and Drosha, and the second by a complex of TRBP and Dicer. dgcr8Δ/Δ mouse embryonic stem cells (mESCs) have less severe phenotypes than dicer1Δ/Δ mESCs, suggesting a physiological role for Microprocessor-independent, Dicer-dependent small RNAs. To identify these small RNAs with unusual biogenesis, we performed high-throughput sequencing from wild-type, dgcr8Δ/Δ, and dicer1Δ/Δ mESCs. Several of the resulting DGCR8-independent, Dicer-dependent RNAs were noncanonical miRNAs. These derived from mirtrons and a newly identified subclass of miRNA precursors, w...
Subjects
free text keywords: Developmental Biology, Genetics, Research Paper
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