publication . Article . Other literature type . 2011

Alterations of gyrA, gyrB, and parC and Activity of Efflux Pump in Fluoroquinolone-resistant Acinetobacter baumannii

Park, Sunok; Lee, Kyeong Min; Yoo, Yong Sun; Yoo, Jung Sik; Yoo, Jae Il; Kim, Hwa Su; Lee, Yeong Seon; Chung, Gyung Tae;
  • Published: 01 Dec 2011
  • Publisher: E-Tree Publishing
Abstract
Objectives This study investigated the fluoroquinolone-resistant mechanism of 56 clinical cases of A baumannii infection from 23 non-tertiary hospitals, collected between 2004 and 2006. Methods Susceptibility testing was performed by broth microdilution and Epsilometer test. Analyses of quinolone resistance-determining region (QRDR) were done by sequencing. The activity of the efflux pump was measured using inhibitors. Results The sequences from selected 56 isolates were divided into seven groups (I-VII) on the basis of mutations in gyrA (S83L), parC (S80L, S80W and S84K) and gyrB (containing the novel mutations E679D, D644Y and A677V). The 27 isolates with trip...
Subjects
Medical Subject Headings: biochemical phenomena, metabolism, and nutritionbacterial infections and mycosesbacteriaheterocyclic compounds
free text keywords: Public Health, Environmental and Occupational Health, Infectious Diseases, Efflux, Medicine, business.industry, business, Microbiology, Acinetobacter baumannii, biology.organism_classification, biology, A baumannii, efflux pump fluoroquinolone resistance, gyrA, gyrB, parC, Articles
25 references, page 1 of 2

Perez, F, Hujer, AM, Hujer, KM. Global challenge of multidrug-resistant Acinetobacter baumannii.. Antimicrob Agents Chemother. 2007; 51 (10): 3471-84 [OpenAIRE] [PubMed]

Valentine, SC, Contreras, D, Tan, S. Phenotypic and molecular characterization of Acinetobacter baumannii clinical isolates from nosocomial outbreaks in Los Angeles County, California.. J Clin Microbiol. 2008; 46 (8): 2499-507 [OpenAIRE] [PubMed]

Spence, RP, Towner, KJ. Frequencies and mechanisms of resistance to moxifloxacin in nosocomial isolates of Acinetobacter baumannii.. J Antimicrob Chemother. 2003; 52 (4): 687-90 [PubMed]

Vila, J, Ruiz, J, Goῇi, P, Marcos, A, Jimenez de Anta, T. Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii.. Antimicrob Agents Chemother. 1995; 39 (5): 1201-3 [OpenAIRE] [PubMed]

Vila, J, Ruiz, J, Goῇi, P, Jimenez de Anta, T. Quinolone-resistance mutations in the topoisomerase IV parC gene of Acinetobacter baumannii.. J Antimicrob Chemother. 1997; 39 (6): 757-62 [OpenAIRE] [PubMed]

Hamouda, A, Amyes, SGB. Novel gyrA and parC point mutations in two strains of Acinetobacter baumannii resistant to ciprofloxacin.. J Antimicrob Chemother. 2004; 54 (3): 695-6 [OpenAIRE] [PubMed]

Ruiz, J. Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection.. J Antimicrob Chemother. 2003; 51 (5): 1109-17 [PubMed]

Higgins, PG, Wisplinghoff, H, Stefanik, D, Seifert, H. Selection of topoisomerase mutaitons and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii.. J Antimicrob Chemother. 2004; 54 (4): 821-3 [PubMed]

Vila, J, Ribera, A, Marco, F. Activity of clinafloxacin, compared with six other quinolones, against Acinetobacter baumannii clinical isolates.. J Antimicrob Chemother. 2002; 49 (3): 471-7 [PubMed]

Seral, C, Barcia-Macay, M, Mingeot-Leclercq, MP. Comparative activity of quinolones (ciprofloxacin, levofloxacin, moxifloxacin and garenoxacin) against extracellular and intracellular infection by Listeria monocytogenes and Staphylococcus aureus in J774 macrophages.. J Antimicrob Chemother. 2005; 55 (4): 511-7 [OpenAIRE] [PubMed]

Noble, CG, Maxwell, A. The role of GyrB in the DNA cleavagereligation reaction of DNA Gyrase: a proposed two metal-ion mechanism.. J Mol Biol. 2002; 318 (2): 361-71 [PubMed]

Heddle, J, Maxwell, A. Quinolone-binding pocket of DNA gyrase: role of GyrB.. Antimicrob Agents Chemother. 2002; 46 (6): 1805-15 [OpenAIRE] [PubMed]

Lee, K, Chang, CL, Lee, NY. Korean Nationwide Surveillance of Antimicriobial Resistance of Bacteria in 1998.. Yonsei Med J. 2000; 41 (4): 497-506 [PubMed]

Yong, D, Cheong, HJ, Kim, YS. In vitro activity of gemifloxacin against recent clinical isolates of bacteria in Korea.. J Koran Med Sci. 2002; 17 (6): 737-42

Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard - 7th ed.. 2007: M100-S20

25 references, page 1 of 2
Abstract
Objectives This study investigated the fluoroquinolone-resistant mechanism of 56 clinical cases of A baumannii infection from 23 non-tertiary hospitals, collected between 2004 and 2006. Methods Susceptibility testing was performed by broth microdilution and Epsilometer test. Analyses of quinolone resistance-determining region (QRDR) were done by sequencing. The activity of the efflux pump was measured using inhibitors. Results The sequences from selected 56 isolates were divided into seven groups (I-VII) on the basis of mutations in gyrA (S83L), parC (S80L, S80W and S84K) and gyrB (containing the novel mutations E679D, D644Y and A677V). The 27 isolates with trip...
Subjects
Medical Subject Headings: biochemical phenomena, metabolism, and nutritionbacterial infections and mycosesbacteriaheterocyclic compounds
free text keywords: Public Health, Environmental and Occupational Health, Infectious Diseases, Efflux, Medicine, business.industry, business, Microbiology, Acinetobacter baumannii, biology.organism_classification, biology, A baumannii, efflux pump fluoroquinolone resistance, gyrA, gyrB, parC, Articles
25 references, page 1 of 2

Perez, F, Hujer, AM, Hujer, KM. Global challenge of multidrug-resistant Acinetobacter baumannii.. Antimicrob Agents Chemother. 2007; 51 (10): 3471-84 [OpenAIRE] [PubMed]

Valentine, SC, Contreras, D, Tan, S. Phenotypic and molecular characterization of Acinetobacter baumannii clinical isolates from nosocomial outbreaks in Los Angeles County, California.. J Clin Microbiol. 2008; 46 (8): 2499-507 [OpenAIRE] [PubMed]

Spence, RP, Towner, KJ. Frequencies and mechanisms of resistance to moxifloxacin in nosocomial isolates of Acinetobacter baumannii.. J Antimicrob Chemother. 2003; 52 (4): 687-90 [PubMed]

Vila, J, Ruiz, J, Goῇi, P, Marcos, A, Jimenez de Anta, T. Mutation in the gyrA gene of quinolone-resistant clinical isolates of Acinetobacter baumannii.. Antimicrob Agents Chemother. 1995; 39 (5): 1201-3 [OpenAIRE] [PubMed]

Vila, J, Ruiz, J, Goῇi, P, Jimenez de Anta, T. Quinolone-resistance mutations in the topoisomerase IV parC gene of Acinetobacter baumannii.. J Antimicrob Chemother. 1997; 39 (6): 757-62 [OpenAIRE] [PubMed]

Hamouda, A, Amyes, SGB. Novel gyrA and parC point mutations in two strains of Acinetobacter baumannii resistant to ciprofloxacin.. J Antimicrob Chemother. 2004; 54 (3): 695-6 [OpenAIRE] [PubMed]

Ruiz, J. Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection.. J Antimicrob Chemother. 2003; 51 (5): 1109-17 [PubMed]

Higgins, PG, Wisplinghoff, H, Stefanik, D, Seifert, H. Selection of topoisomerase mutaitons and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii.. J Antimicrob Chemother. 2004; 54 (4): 821-3 [PubMed]

Vila, J, Ribera, A, Marco, F. Activity of clinafloxacin, compared with six other quinolones, against Acinetobacter baumannii clinical isolates.. J Antimicrob Chemother. 2002; 49 (3): 471-7 [PubMed]

Seral, C, Barcia-Macay, M, Mingeot-Leclercq, MP. Comparative activity of quinolones (ciprofloxacin, levofloxacin, moxifloxacin and garenoxacin) against extracellular and intracellular infection by Listeria monocytogenes and Staphylococcus aureus in J774 macrophages.. J Antimicrob Chemother. 2005; 55 (4): 511-7 [OpenAIRE] [PubMed]

Noble, CG, Maxwell, A. The role of GyrB in the DNA cleavagereligation reaction of DNA Gyrase: a proposed two metal-ion mechanism.. J Mol Biol. 2002; 318 (2): 361-71 [PubMed]

Heddle, J, Maxwell, A. Quinolone-binding pocket of DNA gyrase: role of GyrB.. Antimicrob Agents Chemother. 2002; 46 (6): 1805-15 [OpenAIRE] [PubMed]

Lee, K, Chang, CL, Lee, NY. Korean Nationwide Surveillance of Antimicriobial Resistance of Bacteria in 1998.. Yonsei Med J. 2000; 41 (4): 497-506 [PubMed]

Yong, D, Cheong, HJ, Kim, YS. In vitro activity of gemifloxacin against recent clinical isolates of bacteria in Korea.. J Koran Med Sci. 2002; 17 (6): 737-42

Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, approved standard - 7th ed.. 2007: M100-S20

25 references, page 1 of 2
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publication . Article . Other literature type . 2011

Alterations of gyrA, gyrB, and parC and Activity of Efflux Pump in Fluoroquinolone-resistant Acinetobacter baumannii

Park, Sunok; Lee, Kyeong Min; Yoo, Yong Sun; Yoo, Jung Sik; Yoo, Jae Il; Kim, Hwa Su; Lee, Yeong Seon; Chung, Gyung Tae;