publication . Other literature type . Article . 2012

Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin B as a candidate pancreatic cancer biomarker

Ray, Partha; Rialon-Guevara, Kristy L.; Veras, Emanuela; Sullenger, Bruce A.; White, Rebekah R.;
Open Access English
  • Published: 01 May 2012
  • Publisher: American Society for Clinical Investigation
Abstract
Most cases of pancreatic cancer are not diagnosed until they are no longer curable with surgery. Therefore, it is critical to develop a sensitive, preferably noninvasive, method for detecting the disease at an earlier stage. In order to identify biomarkers for pancreatic cancer, we devised an in vitro positive/negative selection strategy to identify RNA ligands (aptamers) that could detect structural differences between the secretomes of pancreatic cancer and non-cancerous cells. Using this molecular recognition approach, we identified an aptamer (M9-5) that differentially bound conditioned media from cancerous and non-cancerous human pancreatic cell lines. This...
Subjects
free text keywords: Technical Advance, General Medicine, Biochemistry, Negative selection, Pancreatic cancer, medicine.disease, medicine, Biomarker (medicine), Proteome, SELEX Aptamer Technique, Molecular biology, Cancer research, business.industry, business, Adenocarcinoma, Pancreas, medicine.anatomical_structure, Aptamer
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