publication . Other literature type . Article . 2001

Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

E. Tomaszewski; V. G. Wilson; W. L. Wigle; D. N. Phalen;
Open Access English
  • Published: 01 Feb 2001
  • Publisher: American Society for Microbiology
Abstract
Pacheco's disease (PD) is a common, often fatal, disease of parrots. We cloned a virus isolate from a parrot that had characteristic lesions of PD. Three viral clones were partially sequenced, demonstrating that this virus was an alphaherpesvirus most closely related to the gallid herpesvirus 1. Five primer sets were developed from these sequences. The primer sets were used with PCR to screen tissues or tissue culture media suspected to contain viruses from 54 outbreaks of PD. The primer sets amplified DNA from all but one sample. Ten amplification patterns were detected, indicating that PD is caused by a genetically heterogeneous population of viruses. A single...
Subjects
Medical Subject Headings: viruses
free text keywords: Clinical Veterinary Microbiology, Polymerase chain reaction, law.invention, law, Genetics, Viral disease, Herpesviridae, medicine.disease_cause, medicine, Gallid herpesvirus 1, biology.organism_classification, biology, Population, education.field_of_study, education, Virology, Genetic heterogeneity, Virus, Primer (molecular biology)
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