publication . Article . Other literature type . 2013

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Barry J. Beaty; Carol D. Blair; Irma Sanchez-Vargas; Lars Eisen; Natalia V. Voge;
Open Access
  • Published: 06 Feb 2013 Journal: The American Journal of Tropical Medicine and Hygiene, volume 88, pages 260-266 (issn: 0002-9637, eissn: 1476-1645, Copyright policy)
  • Publisher: American Society of Tropical Medicine and Hygiene
Abstract
Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed in...
Subjects
Medical Subject Headings: virusesvirus diseasesbiochemical phenomena, metabolism, and nutritionparasitic diseasesfungi
free text keywords: Parasitology, Virology, Infectious Diseases, Articles, Aedes, biology.organism_classification, biology, Dengue virus, medicine.disease_cause, medicine, Dengue fever, medicine.disease, Aedes aegypti, Vector (epidemiology), Dengue virus NS1 Antigen, Elisa kit, Virus isolation
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