publication . Article . Other literature type . 2006

Chromosome Conformation Capture Carbon Copy (5C): A massively parallel solution for mapping interactions between genomic elements

Chad Nusbaum; Job Dekker; Anton Krumm; Roland D. Green; Tracey Honan; Ramy Arnaout; Eric D. Rubio; Justin Lamb; William Lee; Josée Dostie; ...
Open Access
  • Published: 01 Oct 2006 Journal: Genome Research, volume 16, pages 1,299-1,309 (issn: 1088-9051, Copyright policy)
  • Publisher: Cold Spring Harbor Laboratory
Abstract
Physical interactions between genetic elements located throughout the genome play important roles in gene regulation and can be identified with the Chromosome Conformation Capture (3C) methodology. 3C converts physical chromatin interactions into specific ligation products, which are quantified individually by PCR. Here we present a high-throughput 3C approach, 3C-Carbon Copy (5C), that employs microarrays or quantitative DNA sequencing using 454-technology as detection methods. We applied 5C to analyze a 400-kb region containing the human β-globin locus and a 100-kb conserved gene desert region. We validated 5C by detection of several previously identified loop...
Subjects
free text keywords: Methods, DNA microarray, Locus (genetics), Locus control region, Biology, Genomics, Chromosome conformation capture, Genetics, Genome, DNA sequencing, Chromosome
Any information missing or wrong?Report an Issue