publication . Article . Other literature type . 2000

The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains.

Kay, B. K.; Mike Williamson; Sudol, M.;
Restricted
  • Published: 01 Feb 2000 Journal: The FASEB Journal, volume 14, pages 231-241 (issn: 0892-6638, eissn: 1530-6860, Copyright policy)
  • Publisher: Wiley
Abstract
Acommon focus among molecular and cellular biologists is the identification of proteins that interact with each other. Yeast two-hybrid, cDNA expression library screening, and coimmunoprecipitation experiments are powerful methods for identifying novel proteins that bind to one’s favorite protein for the purpose of learning more regarding its cellular function. These same techniques, coupled with truncation and mutagenesis experiments, have been used to define the region of interaction between pairs of proteins. One conclusion from this work is that many interactions occur over short regions, often less than 10 amino acids in length within one protein. For examp...
Subjects
free text keywords: Biotechnology, Genetics, Biochemistry, Molecular Biology, Computational biology, Immunoprecipitation, Binding site, Biology, PDZ domain, Amino acid, chemistry.chemical_classification, chemistry, Endocrinology, medicine.medical_specialty, medicine, Phosphotyrosine binding, Postsynaptic density, Internal medicine, Plasma protein binding, Complementary DNA
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue