Specific direct fluorescent antibody identification of Mycobacterium leprae.

E, Matsuo; O K, Skinsnes

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International Journal of Leprosy and Other Mycobacterial Diseases

Article . 1976

Data sources: Europe PubMed Central

Specific direct fluorescent antibody identification of Mycobacterium leprae.

descriptionPublicationkeyboard_double_arrow_right Article 02 Jun 1976 English Journal:International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association, volume 43 (issn: 0148-916X,

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Authors: E, Matsuo; O K, Skinsnes;

pmid: 767263

Specific direct fluorescent antibody identification of Mycobacterium leprae.

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Abstract

In vitro cultivation of M. leprae requires a rapid, specific identification procedure for monitoring the cultures. A method utilizing direct FITC-coupled lepromatous, specific serum globulin is described in detail with suggestions for improvement. After various purification and adsorption procedures, notably against human liver powder and M. tuberculosis, a fluorescent serum preparation is obtained which specifically reacts with M. leprae and not with other mycobacteria.

Keywords

Mycobacterium leprae, Antibody Specificity, Fluorescent Antibody Technique, Serum Globulins, Antibodies, Bacterial, Culture Media

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