Cytological effects of culture media conditioned by B16 melanoma cells and 3T3 fibroblasts.
Copyright policy )Cytological effects of culture media conditioned by B16 melanoma cells and 3T3 fibroblasts.
Culture media conditioned (CM) by mixed populations of mouse B16 melanoma cells and 3T3 fibroblasts cultivated as monolayers exert cytological effects on B16 or 3T3 cells when treated separately in culture. By ultrafiltration of these CM, we show that a stimulatory activity on B16 melanoma cells proliferation is present in fractions with M.W. greater than 10,000 daltons. A strong cytotoxic activity for B16 melanoma cells and, to a lower degree, for 3T3 fibroblasts is detected in fractions with M.W. less than 1,000 daltons. The ultrastructural analysis of cells (B16 or 3T3) treated with cytotoxic fractions reveals in them mitochondrial swelling, blebs, broken membranes and dead cells.
- University of Liège Belgium
3T3 fibroblasts, Anatomie (cytologie, histologie, embryologie...) & physiologie, Cell Survival, Melanoma, Experimental, Ultrafiltration, Fibroblasts, Anatomy (cytology, histology, embryology...) & physiology, Life sciences, Growth Inhibitors, Cell Line, Culture Media, Mice, Inbred C57BL, Molecular Weight, Kinetics, Mice, conditoned media, mitochondria swelling, Culture Techniques, Sciences du vivant, B16 melanoma, Animals, Mitogens
3T3 fibroblasts, Anatomie (cytologie, histologie, embryologie...) & physiologie, Cell Survival, Melanoma, Experimental, Ultrafiltration, Fibroblasts, Anatomy (cytology, histology, embryology...) & physiology, Life sciences, Growth Inhibitors, Cell Line, Culture Media, Mice, Inbred C57BL, Molecular Weight, Kinetics, Mice, conditoned media, mitochondria swelling, Culture Techniques, Sciences du vivant, B16 melanoma, Animals, Mitogens
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