publication . Article . 2014

Cloning of the GH gene from the beluga sturgeon (Huso huso) into a lentivial & none viral constructs and it’s expression in HEK cell lines

Mashjoor, S.; Zolgharnain, H.; Gardaneh, M.; Salari Aliabadi, M.A.; Qasemi, A.; Azimi, Z.;
Open Access Persian
  • Published: 01 Jan 2014
Abstract
Caviar-producing fish with their economically valuable product are important in fisheries. The cDNA growth hormone (GH) of Beluga sturgeon (Huso huso) was constructed using total RNA from pituitary glands. To construct the recombinant and active lentiviruses carring GH gene, this DNA sequence was inserted into the cloning vector pTZ57R/T and subsequently cutted from pTZ57R/T by endonuclease enzyme and incorporated into lentivirus vector pNL-EGFP/CMV-WPRE on upstream of an IRES cassette. We also insert a reporter EGFP gene downstream of IRES so transfection and transduction steps can be traced. Using this vector plus virus packaging and envelope vectors, HEK293T ...
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free text keywords: Aquaculture, Biology, Biology, Hormone, Growth, Huso huso, Lentivirus, Gene Expression, Transgene, Beluga sturgeon, Fish, Iran, Aquaculture, Growth hormone
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Fisheries and Aquaculture Management
European Marine Science
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