Magnolia × pruhoniciana belongs to the most extraordinary biomass in terms of its exceptional character. It is a unique hybrid originated by the crossing of Japanese M. obovata and American M. tripetala. It has been cultivated and grown since the 1950s in the Průhonice Park, part of the Institute of Botany of the CAS. Nevertheless, biologically active substances contained in intensely fragrant blossoms have not been sufficiently analysed yet. For this reason, the\nresearch was focused on the extraction of volatile substances from fresh blossoms using various solvents, which markedly influenced the profiles of volatile substances, alkaloids, coumarins, flavonoids, lignans and neolignans, phenyl-propanoids and terpenes etc. Magnolol (2 to 11%) and honokiol (0.3 to 4.6%) were initially described as components of the genus Magnolia, which are components of Chinese (Kampo) herbs. The extracts of magnolia flowers showed anti-oxidative, anti-inflammatory, anti-tumour, anti-diabetic complications, anti-microbial, anti-neurodegenerative, anti-depressant, anti-nociceptive, hormone, gastrointestinal, and uterus modulation, cardiovascular and liver protective properties.