62 pages ; Microalgae have great potential in closing the carbon cycle. They fix nearly 40% of atmospheric CO2 and have various applications in fields of energy, food and medicine. However, the scale-up of the conventional microalgae cultivation has been limited by the needs for massive land use, freshwater resources, and energy consumption. This research aims to increase algal biomass yield by enhancing CO2 capture from air using regenerable materials and acclimating Chlorella vulgaris for growth in wastewater and salt water. Novel synthetic buoyant calcium alginate beads encapsulated using amino acid salts such as sodium glycinate (Na-Gly), sodium arginate (Na-Arg), and sodium salt of l-lysine (Na-Ly) are synthesized for direct air capture of CO2 for enhanced microalgae growth. These synthesized beads increase biomass growth of Chlorella vulgaris by 235%~274% compared with the control group. The beads with 0.1 % Na-Arg and Na-Ly mixture show the highest efficiency in accelerating microalgae biomass growth, and the estimated carbon fixation rate of this system is 0.32 g/L/day. A key consideration is the synthesis of the functionalized materials to prevent the leakage of chemicals which can decrease the growth rate of algal biomass. The beads have reticular structure mainly composed of calcium alginate polymers, which are attached with CO2 absorbents by glutaraldehyde (GA). Amino acids residuals from the attached CO2 absorbents react with CO2, which leads to the accelerated CO2 fixation. Future work is oriented towards exploring various strategies for increasing the stability of the functional groups on buoyant beads to increase the reuse of these materials over multiple cycles of direct air capture and algae growth.