Recent toxicology, in accordance with recommendations from the European Council, has demanded decrease in the number of vertebrates used in toxicology testing and their partial replacement with invertebrate animals, plants or even organ, tissue, or cell cultures. During the last 50 years various invertebrate species have been tested for their sensitivity to many chemical or physical agents to prove their possible use for pre-screening tests. To the most valuable organisms available for ecotoxicity testing belong crustaceans of the Artemia genus, commonly known as brine shrimps.