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Chain Sliding versus β-Sheet Formation upon Shearing Single α-Helical Coiled Coils

Authors: Anna-Maria Tsirigoni; Melis Goktas; Kerstin G. Blank;

Chain Sliding versus β-Sheet Formation upon Shearing Single α-Helical Coiled Coils

Abstract

This version provides an additional zipped folder (16_SMFS_Chainfits) that includes the fitted rupture forces and loading rates for all selected force-extension curves, measured at the respective retract speeds. These files were not included in the previous version. The ReadMe file has been updated accordingly. This library contains the circular dichroism (CD) spectra as well as the data from CD thermal denaturation (TD) experiments. It further contains atomic force microscope (AFM)-based single-molecule force spectroscopy (SMFS) data, specifically the selected force-extension curves used for Bell-Evans analysis. The SMFS raw data are published as separate Zenodo entries (see below). Please find additional information also in the supplementary material of the article. The raw SMFS data (no selection of force-extension curves) are published in .txt format as separate uploads: A4N3B4N3 (A4B4): 10.5281/zenodo.7681437 A5N3B5N3 (A5B5): 10.5281/zenodo.7682163 A6N35B6N35 (A6B6): 10.5281/zenodo.7682201 A6IB6I (A4*B4): 10.5281/zenodo.7682223

Keywords

alpha-helix, protein mechanics, beta-sheet, single-molecule force spectroscopy, coiled coils

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