Objectives: To assess the frequency of UTI causing organisms and to determine the frequency in both the genders. Materials and Methods: The design of this study was a cross sectional study which was carried out at Kulsoom International Hospital, Islamabad and the duration of this study was from July 2019 to June 2020. The patients presented at the urology department with complaints of urinary tract infection aging 21-70 years were enrolled with simple purposive sampling in the study. The majority of the patients got enrolled were aging 21-35 years. The data was collected with the help of preformed questionnaire. Results: 340 patients were enrolled in the study 202 females and 138 males by purposive sampling. The urine sample was collected and sent to hospital laboratory for culture and examination. most of cases with positive culture with identified organisms had klebsiella pneumonia, Staphylococcus sp., Escherichia Coli, Pseudomonas, Proteus SP and Candida’s. Conclusions: it is concluded from the results of the study that E. coli is the most common organism causing UTI in both males and females. KEYWORDS: Urinary tract infections, isolated microorganisms, Patients.