Background: In the present study we have selected Pterocarpus santalinus (Leaves) which have been utilized by the villager for acne or the other skin disorders. In this view the survey of literature has been carried out and in Ayurveda, it has been mentioned that this drug is useful in skin disease. Hence, the present work has been selected to evaluate the anti acne activity and scientific validity to traditional claims. Methods: standard test and experimental procedure was used for the estimation of TPC, TFC and evaluation of Anti-Acne activities of Pterocarpus santalinus leaves extract was studied. The acne like inflammatory model was produced in the ears of male Sprague Dawly rats (180-220g) by subcutaneous injection of heat-killed bacteria Propionibacterium acnes (650C for 30 min) which was evaluated by measuring ear thickness (vernier calliper) and % inhibition of acne in different groups. Results: The total phenols and flavonoids were found to be 18.01 and 25.14 mcg/ml respectively. The Hydroalcoholic extract of Pterocarpus santalinus leaves showeddecrease in ear thickness and % inhibition at dose dependent manner(200 and 300 mg/kg). Conclusions: The presented data indicate that the administration of Hydroalcoholic extract of Pterocarpus santalinus (Leaves) at dose of 200 and 300mg/kg decreased inflammation and showed antibacterial activity. The Hydroalcoholic extract of Pterocarpus santalinus (Leaves) have potent anti-acne activity.