publication . Article . 2021

NEW HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF EMTRICITABINE IN MARKETED FORMULATION

Prabhat Tiwari, Dr. Alok Pal Jain, Vivek Shrivastava;
Open Access
  • Published: 11 Nov 2021
  • Publisher: Zenodo
Abstract
A novel stability-indicating RP-HPLC method has been developed and validated for quantitative analysis of Emtricitabine in the bulk drug and in a pharmaceutical dosage form. An isocratic separation of EMT was achieved on Thermo C18 column (4.6 x 250mm, 5 μ particle size) as the stationary phase with a flow rate of 1 ml/min and using a UV detector to monitor the eluate at 254 nm. The mobile phase consisted of Acetonitrile: methanol (50:50v/v) enabled separation of the drug from its degradation products. The method was validated for linearity, accuracy (recovery), precision, specificity, and robustness. Linearity was established by least squares linear regression ...
Any information missing or wrong?Report an Issue